Sun Qingqing, Li Jinyu, Cheng Wenzhen, Guo Huihong, Liu Xiaomin, Gao Hongbo
College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China.
Genes (Basel). 2018 May 17;9(5):257. doi: 10.3390/genes9050257.
Purple acid phosphatases (PAPs) play various physiological roles in plants. AtPAP2 was previously shown to localize to both chloroplasts and mitochondria and to modulate carbon metabolism in . Over-expression of AtPAP2 resulted in faster growth and increased biomass in several plant species, indicating its great potential for crop improvement of phosphate use and yield. Here, we studied the localization of AtPAP2 by transient expression in tobacco leaves. The results showed AtPAP2 was localized to the plasma membrane through the secretory pathway, which is different from previous studies. We also found that AtPAP2 had a close relationship with fungal PAP2-like proteins based on phylogenetic analysis. In addition, the C-terminal transmembrane domain conserved in land plants is unique among other AtPAPs except AtPAP9, which is a close homolog of AtPAP2. Taken together, our results provide information for further study of AtPAP2 in understanding its special function in crop improvement.
紫色酸性磷酸酶（PAPs）在植物中发挥着多种生理作用。先前研究表明，AtPAP2定位于叶绿体和线粒体，并调节碳代谢。AtPAP2的过表达导致几种植物物种生长加快、生物量增加，表明其在改善作物磷利用和产量方面具有巨大潜力。在此，我们通过在烟草叶片中瞬时表达来研究AtPAP2的定位。结果表明，AtPAP2通过分泌途径定位于质膜，这与先前的研究不同。基于系统发育分析，我们还发现AtPAP2与真菌PAP2样蛋白有密切关系。此外，陆地植物中保守的C端跨膜结构域在除AtPAP9之外的其他AtPAP中是独特的，AtPAP9是AtPAP2的紧密同源物。综上所述，我们的结果为进一步研究AtPAP2以了解其在作物改良中的特殊功能提供了信息。
