亲水作用液相色谱串联质谱法测定薄荷属植物、罗勒属植物及薰衣草属植物叶片中的氨基酸

Hilic MS/MS determination of amino acids in herbs of L., L., and leaves of L.

作者信息

Prokopenko Yuliya, Jakštas Valdas, Žvikas Vaidotas, Georgiyants Victoriya, Ivanauskas Liudas

机构信息

a Department of Quality, Standardisation and Certification of Drugs , National University of Pharmacy , Kharkiv , Ukraine.

b Faculty of Pharmacy, Medical Academy, Department of Pharmacognosy , Lithuanian University of Health Sciences , Kaunas , Lithuania.

出版信息

Nat Prod Res. 2019 Jul;33(13):1961-1963. doi: 10.1080/14786419.2018.1477145. Epub 2018 May 18.

DOI:10.1080/14786419.2018.1477145

PMID:29772944

Abstract

The aim of research was to study the content of amino acids using in extracts of L., L., and L. by HILIC MS/MS method. Separation of amino acids in the samples was carried out with Acquity H-class UPLC system (Waters, Milford, USA) equipped with SeQuant ZIC-Hilic collumn (2.1 × 150 mm, 3.5 μm) (Merck Millipore, Darmstadt, Germany). The MS/MS fragment ion chromatograms of the test solutions established the presence of 19 amino acids. The obtained results have shown that L. characterized the highest concentrations of different neurogenic amino acids (128.1 mg/kg), comparing with and (57.72 and 52.91 mg/kg, respectively).

摘要

本研究的目的是采用亲水作用色谱串联质谱法（HILIC MS/MS）研究[植物名称1]、[植物名称2]和[植物名称3]提取物中的氨基酸含量。样品中的氨基酸分离采用配备了SeQuant ZIC-Hilic柱（2.1×150 mm，3.5μm）（德国默克密理博公司，达姆施塔特）的Acquity H-class超高效液相色谱系统（美国沃特世公司，米尔福德）。测试溶液的MS/MS碎片离子色谱图证实了19种氨基酸的存在。所得结果表明，与[植物名称2]和[植物名称3]（分别为57.72和52.91 mg/kg）相比，[植物名称1]中不同神经源性氨基酸的浓度最高（128.1 mg/kg）。