罗勒变种玛利亚博尼塔乙醇粗提物和己烷馏分的抗隐球菌活性：作用机制及与两性霉素B和罗勒精油的协同作用
Anti-cryptococcal activity of ethanol crude extract and hexane fraction from Ocimum basilicum var. Maria bonita: mechanisms of action and synergism with amphotericin B and Ocimum basilicum essential oil.
作者信息
Cardoso Nathalia N R, Alviano Celuta S, Blank Arie F, Arrigoni-Blank Maria de Fátima, Romanos Maria Teresa V, Cunha Marcel M L, da Silva Antonio Jorge R, Alviano Daniela S
机构信息
a Department of General Microbiology , Institute of Microbiology Paulo de Góes, Federal University of Rio de Janeiro , Rio de Janeiro , Brazil.
b Postgraduate Program in Plant Biotechnology , Federal University of Rio de Janeiro , Rio de Janeiro , Brazil.
出版信息
Pharm Biol. 2017 Dec;55(1):1380-1388. doi: 10.1080/13880209.2017.1302483.
CONTEXT
Ocimum basilicum L. (Lamiaceae) has been used in folk medicine to treat headaches, kidney disorders, and intestinal worms.
OBJECTIVE
This study evaluates the anti-cryptococcal activity of ethanol crude extract and hexane fraction obtained from O. basilicum var. Maria Bonita leaves.
MATERIALS AND METHODS
The MIC values for Cryptococcus sp. were obtained according to Clinical and Laboratory Standards Institute in a range of 0.3-2500 μg/mL. The checkerboard assay evaluated the association of the substances tested (in a range of 0.099-2500 μg/mL) with amphotericin B and O. basilicum essential oil for 48 h. The ethanol extract, hexane fraction and associations in a range of 0.3-2500 μg/mL were tested for pigmentation inhibition after 7 days of treatment. The inhibition of ergosterol synthesis and reduction of capsule size were evaluated after the treatment with ethanol extract (312 μg/mL), hexane fraction (78 μg/mL) and the combinations of essential oil + ethanol extract (78 μg/mL + 19.5 μg/mL, respectively) and essential oil + hexane fraction (39.36 μg/mL + 10 μg/mL, respectively) for 24 and 48 h, respectively.
RESULTS
The hexane fraction presented better results than the ethanol extract, with a low MIC (156 μg/mL against C. neoformans T and 312 μg/mL against C. neoformans H99 serotype A and C. gattii WM779 serotype C). The combination of the ethanol extract and hexane fraction with amphotericin B and essential oil enhanced their antifungal activity, reducing the concentration of each substance needed to kill 100% of the inoculum. The substances tested were able to reduce the pigmentation, capsule size and ergosterol synthesis, which suggest they have important mechanisms of action.
CONCLUSIONS
These results provide further support for the use of ethanol extracts of O. basilicum as a potential source of antifungal agents.
背景
罗勒（唇形科）在民间医学中被用于治疗头痛、肾脏疾病和肠道寄生虫。
目的
本研究评估了从玛利亚博妮塔罗勒叶中获得的乙醇粗提物和己烷馏分的抗隐球菌活性。
材料与方法
根据临床和实验室标准协会的方法，获得隐球菌属的最低抑菌浓度（MIC）值，范围为0.3 - 2500μg/mL。棋盘法评估了受试物质（浓度范围为0.099 - 2500μg/mL）与两性霉素B和罗勒精油联合作用48小时的效果。在处理7天后，测试乙醇提取物、己烷馏分以及浓度范围为0.3 - 2500μg/mL的联合物对色素沉着的抑制作用。在用乙醇提取物（312μg/mL）、己烷馏分（78μg/mL）以及精油 + 乙醇提取物（分别为78μg/mL + 19.5μg/mL）和精油 + 己烷馏分（分别为39.36μg/mL + 10μg/mL）处理24小时和48小时后，评估对麦角固醇合成的抑制作用和荚膜大小的减小情况。
结果
己烷馏分的效果优于乙醇提取物，其MIC较低（对新型隐球菌T为156μg/mL，对新型隐球菌H99血清型A和加氏隐球菌WM779血清型C为312μg/mL）。乙醇提取物和己烷馏分与两性霉素B和精油的联合增强了它们的抗真菌活性，降低了杀死100%接种物所需的每种物质的浓度。受试物质能够减少色素沉着、荚膜大小和麦角固醇合成，这表明它们具有重要的作用机制。
结论
这些结果为将罗勒乙醇提取物用作抗真菌剂的潜在来源提供了进一步的支持。
Zotero 插件