Elangovan Perumal, Ramakrishnan Ramalingam, Amudha Kasinathan, Jalaludeen Abdulkadhar Mohamed, Sagaran Gunasekaran Karuna, Babu Frankline Rajan, Pari Leelavinothan
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalai Nagar - 608002, Tamil Nadu, India.
Department of Biochemistry, St. Joseph's College of Arts & Science (Autonomous), Cuddalore 607001 Tamil Nadu, India.
J Environ Pathol Toxicol Oncol. 2018;37(1):1-14. doi: 10.1615/JEnvironPatholToxicolOncol.2017025087.
Nickel (Ni) is an important environmental toxicant that can cause cancer and cardiovascular disease. The aim of this study was to examine the protective effects of troxerutin (Txn) Ni-induced renal dysfunction in rats using biochemical and histopathological approaches. Nickel (20 mg/kg body weight [b.w.]/day) was administered intraperitoneally (i.p.) for 20 days. Renal damage from Ni toxicity was evident from the changed levels of serum and urinary markers in Ni-treated rats. The levels of lipid peroxidation markers also significantly increased, while the levels of nonenzymatic and enzymatic antioxidants significantly decreased in the kidney of Ni-intoxicated rats. Troxerutin was administered orally (100 mg/kg b.w.) for 20 days along with Ni, resulting in a reversal of Ni-induced biochemical changes in kidney accompanied by a significant decrease in lipid peroxidation and an increase in the level of renal antioxidant defense system. Histopathological studies in the kidneys of rats also showed that troxerutin (100 mg/ kg b.w.) markedly reduced the toxicity of Ni and preserved the normal histological architecture of the renal tissue. The present study results suggest the nephroprotective potential of Txn in Ni toxicity, which might be due to its antioxidant and metal-chelating properties.
镍（Ni）是一种重要的环境毒物，可导致癌症和心血管疾病。本研究的目的是采用生化和组织病理学方法，研究曲克芦丁（Txn）对镍诱导的大鼠肾功能障碍的保护作用。腹腔注射（i.p.）给予镍（20毫克/千克体重[b.w.]/天），持续20天。从镍处理大鼠血清和尿液标志物水平的变化可以明显看出镍毒性对肾脏的损害。脂质过氧化标志物水平也显著升高，而镍中毒大鼠肾脏中非酶和酶抗氧化剂水平显著降低。曲克芦丁与镍同时口服（100毫克/千克b.w.），持续20天，导致镍诱导的肾脏生化变化逆转，脂质过氧化显著降低，肾脏抗氧化防御系统水平升高。对大鼠肾脏的组织病理学研究还表明，曲克芦丁（100毫克/千克b.w.）显著降低了镍的毒性，并保留了肾组织的正常组织结构。本研究结果表明曲克芦丁对镍毒性具有肾保护潜力，这可能归因于其抗氧化和金属螯合特性。
