Meng Fanli, Yang Mingyu, Li Yang, Li Tianyu, Liu Xinxin, Wang Guoyue, Wang Zhanchun, Jin Xianhao, Li Wenbin
Key Laboratory of Soybean Biology in Chinese Ministry of Education, Northeast Agricultural University, Harbin, China.
Front Physiol. 2018 May 3;9:383. doi: 10.3389/fphys.2018.00383. eCollection 2018.
RNA interference (RNAi) is useful for controlling pests of agriculturally important crops. The soybean pod borer (SPB) is the most important soybean pest in Northeastern Asia. In an earlier study, we confirmed that the SPB could be controlled via transgenic plant-mediated RNAi. Here, the SPB transcriptome was sequenced to identify RNAi-related genes, and also to establish an RNAi-of-RNAi assay system for evaluating genes involved in the SPB systemic RNAi response. The core RNAi genes, as well as genes potentially involved in double-stranded RNA (dsRNA) uptake were identified based on SPB transcriptome sequences. A phylogenetic analysis and the characterization of these core components as well as dsRNA uptake related genes revealed that they contain conserved domains essential for the RNAi pathway. The results of the RNAi-of-RNAi assay involving e 2 (a critical cuticle pigmentation gene) as a marker showed that genes encoding the sid-like (), scavenger receptor class C (), and scavenger receptor class B ( and ) proteins of the endocytic pathway were required for SPB cellular uptake of dsRNA. The SPB response was inferred to contain three functional small RNA pathways (i.e., miRNA, siRNA, and piRNA pathways). Additionally, the SPB systemic RNA response may rely on systemic RNA interference deficient transmembrane channel-mediated and receptor-mediated endocytic pathways. The results presented herein may be useful for developing RNAi-mediated methods to control SPB infestations in soybean.
RNA干扰（RNAi）对于控制具有重要农业价值的作物害虫很有用。大豆食心虫（SPB）是东北亚地区最重要的大豆害虫。在早期研究中，我们证实了可以通过转基因植物介导的RNAi来控制SPB。在此，对SPB转录组进行测序以鉴定RNAi相关基因，并建立一个RNAi的RNAi检测系统，用于评估参与SPB系统性RNAi反应的基因。基于SPB转录组序列鉴定了核心RNAi基因以及可能参与双链RNA（dsRNA）摄取的基因。对这些核心成分以及与dsRNA摄取相关基因的系统发育分析和特征描述表明，它们包含RNAi途径必不可少的保守结构域。以e 2（一个关键的表皮色素沉着基因）作为标记的RNAi的RNAi检测结果表明，内吞途径中编码sid样（）、C类清道夫受体（）以及B类清道夫受体（和）蛋白的基因是SPB细胞摄取dsRNA所必需的。推测SPB反应包含三种功能性小RNA途径（即miRNA、siRNA和piRNA途径）。此外，SPB系统性RNA反应可能依赖于系统性RNA干扰缺陷跨膜通道介导的和受体介导的内吞途径。本文给出的结果可能有助于开发RNAi介导的方法来控制大豆中的SPB侵染。
