Luedin Samuel M, Pothier Joël F, Danza Francesco, Storelli Nicola, Frigaard Niels-Ulrik, Wittwer Matthias, Tonolla Mauro
1University of Geneva, Sciences III, Department of Botany and Plant Biology, Microbiology Unit, 1211 Geneva, Switzerland.
University of Applied Sciences of Southern Switzerland (SUPSI), Department of Environment, Constructions and Design (DACD), Laboratory of Applied Microbiology (LMA), Via Mirasole 22A, 6500 Bellinzona, Switzerland.
Stand Genomic Sci. 2018 May 9;13:14. doi: 10.1186/s40793-018-0317-z. eCollection 2018.
" sp. nov. strain Cad16 is a photoautotrophic purple sulfur bacterium belonging to the family of in the class of . The type strain Cad16 was isolated from the chemocline of the alpine meromictic Lake Cadagno in Switzerland. Strain Cad16 represents a key species within this sulfur-driven bacterial ecosystem with respect to carbon fixation. The 7.74-Mbp genome of strain Cad16 has been sequenced and annotated. It encodes 6237 predicted protein sequences and 59 RNA sequences. Phylogenetic comparison based on 16S rRNA revealed that strain DSM 232 the most closely related species. Genes involved in sulfur oxidation, central carbon metabolism and transmembrane transport were found. Noteworthy, clusters of genes encoding the photosynthetic machinery and pigment biosynthesis are found on the 0.48 Mb plasmid pTs485. We provide a detailed insight into the Cad16 genome and analyze it in the context of the microbial ecosystem of Lake Cadagno.
新种菌株Cad16是一种光合自养型紫色硫细菌，属于 纲 目 科。模式菌株Cad16是从瑞士阿尔卑斯山半混合湖卡达诺湖的化学分层带中分离出来的。就碳固定而言，菌株Cad16是这个由硫驱动的细菌生态系统中的关键物种。菌株Cad16的7740kb基因组已被测序和注释。它编码6237个预测的蛋白质序列和59个RNA序列。基于16S rRNA的系统发育比较表明，菌株DSM 232是与其关系最密切的物种。发现了参与硫氧化、中心碳代谢和跨膜运输的基因。值得注意的是，编码光合机构和色素生物合成的基因簇存在于0.48Mb的质粒pTs485上。我们对Cad16基因组进行了详细的研究，并在卡达诺湖微生物生态系统的背景下对其进行了分析。
