Vollmar A M, Friedrich A, Sinowatz F, Schulz R
Institut für Pharmakologie, Toxikologie und Pharmazie, Universität Mäunchen, FRG.
Peptides. 1988 Sep-Oct;9(5):965-71. doi: 10.1016/0196-9781(88)90074-5.
Acidic extracts of guinea pig jejunum and colon contain atrial natriuretic peptide-like material (IR-ANP) detected by radioimmunoassay after purification by Sephadex G-50 gel filtration. Immunohistochemical analysis of guinea pig colon also revealed IR-ANP to be located directly beneath the lamina muscularis mucosae. High performance gel permeation chromatography (HP-GPC) and reverse phase high performance chromatography (RP-HPLC) of the IR-ANP showed correspondence to the 15 kD ANP precursor molecule (pro-ANP). No low molecular weight forms of ANP were detected. The extracted pro-ANP could be converted to alpha-ANP-like material by incubation with serum or supernatant of colonic homogenate. These data indicate the intestine to be a further site of ANP synthesis.
豚鼠空肠和结肠的酸性提取物含有心房利钠肽样物质（免疫反应性心房利钠肽，IR-ANP），经葡聚糖凝胶G-50凝胶过滤纯化后通过放射免疫分析检测到。豚鼠结肠的免疫组织化学分析还显示IR-ANP直接位于黏膜肌层下方。对IR-ANP进行高效凝胶渗透色谱（HP-GPC）和反相高效液相色谱（RP-HPLC）分析，结果显示其与15kD心房利钠肽前体分子（前心房利钠肽，pro-ANP）相对应。未检测到低分子量形式的心房利钠肽。提取的前心房利钠肽与血清或结肠匀浆上清液一起孵育后可转化为α-心房利钠肽样物质。这些数据表明肠道是心房利钠肽合成的另一个部位。
