Matsumara Yasufumi, Peirano Gisele, Kock Marleen, Pitout Johann D D
Kyoto University Graduate School of Medicine, Kyoto, Japan.
Cummings School of Medicine, University of Calgary, #9, 3535 Research Road NW, Calgary, AB, Canada.
Eur J Clin Microbiol Infect Dis. 2025 Feb;44(2):375-381. doi: 10.1007/s10096-024-05010-7. Epub 2024 Dec 11.
Pseudomonas aeruginosa ST111 is a global multidrug resistant (MDR) high-risk clone and comprehensive data about its molecular epidemiology is limited in Canada. Comprehensive data about the evolution of ST111 clades is limited. We characterized a Canadian collection of ST111 causing bloodstream infections and investigated the genomic relationship between Canadian and global ST111.
We used long and short read WGS to characterize Canadian ST111 (n = 10 from 2010-18). We performed phylogenetic analysis on a global collection of ST111 (n = 969) and investigated the evolutionary history of clades using BEAST.
ST111 belonged to 3 clades (A, B, C) and two subclades (C1, C2). ST111-A was the ancestral clade while clades B, C1 and C2 emerged during the 1700s and 1800s. ST111-C2 dominated the global ST111 population. Serotype switching from O4 to O12 and the acquisition of Tn21, gyrA_T83I, parC_S87L, In59 with bla and aacA29 over time, were important in the evolution of ST111-C2. The Calgary ST111 strains consisted of a diverse collection that belonged to ST111-A (O4), ST111-C1 (O4) and ST111-C2 (O12) with different transposon structures.
We provided details on the emergence and evolution of different ST111 clades over time and highlighted the roles of serotype switching and the acquisition of certain AMR determinants and transposon structures in the evolution of ST111-C2.
铜绿假单胞菌ST111是一种全球多重耐药（MDR）高风险克隆，而加拿大关于其分子流行病学的全面数据有限。关于ST111进化枝演变的全面数据也很有限。我们对加拿大一组引起血流感染的ST111进行了特征分析，并研究了加拿大和全球ST111之间的基因组关系。
我们使用长读长和短读长全基因组测序（WGS）来鉴定加拿大的ST111（2010 - 2018年的10株）。我们对全球范围内的ST111菌株（n = 969）进行了系统发育分析，并使用贝叶斯进化分析采样树（BEAST）研究进化枝的进化历史。
ST111属于3个进化枝（A、B、C）和两个亚进化枝（C1、C2）。ST111 - A是祖先进化枝，而进化枝B、C1和C2出现在18世纪和19世纪。ST111 - C2在全球ST111群体中占主导地位。随着时间的推移，血清型从O4转换为O12以及获得Tn21、gyrA_T83I、parC_S87L、携带bla和aacA29的In59，在ST111 - C2的进化中起重要作用。卡尔加里的ST111菌株由不同的菌株组成，它们属于具有不同转座子结构的ST111 - A（O4）、ST111 - C1（O4）和ST111 - C2（O12）。
我们详细阐述了不同ST111进化枝随时间的出现和演变，并强调了血清型转换以及某些抗菌药物耐药性（AMR）决定因素和转座子结构的获得在ST111 - C2进化中的作用。
