DuPont Pioneer, IKP Knowledge Park, Genome Valley, Survey No. 542/2, DS-9, Turkapally Village, Shameerpet Mandal, Ranga Reddy District, Hyderabad, Telangana, 500078, India.
Multi Crop Research Center, DuPont Pioneer, Sy. No. 383, 384 and 385, Tunki Kalsa Village, Wargal Mandal, Medak District, Hyderabad, Telangana, 502 336, India.
Transgenic Res. 2018 Aug;27(4):331-342. doi: 10.1007/s11248-018-0077-y. Epub 2018 May 17.
Genetically modified (GM) crops undergo large scale multi-location field trials to characterize agronomics, composition, and the concentration of newly expressed protein(s) [herein referred to as transgenic protein(s)]. The concentration of transgenic proteins in different plant tissues and across the developmental stages of the plant is considered in the safety assessment of GM crops. Reference or housekeeping proteins are expected to maintain a relatively stable expression pattern in healthy plants given their role in cellular functions. Understanding the effects of genotype, growth stage and location on the concentration of endogenous housekeeping proteins may provide insight into the contribution these factors could have on transgenic protein concentrations in GM crops. The concentrations of three endogenous proteins (actin, elongation factor 1-alpha, and glyceraldehyde 3-phosphate dehydrogenase) were measured in several different maize hybrids grown across multiple field locations over 2 years. Leaf samples were collected from healthy plants at three developmental stages across the growing seasons, and protein concentrations were quantified by indirect enzyme-linked immunosorbent assay (ELISA) for each protein. In general, the concentrations of these three endogenous proteins were relatively consistent across hybrid backgrounds, when compared within one growth stage and location (2-26%CV), whereas the concentrations of proteins in the same hybrid and growth stage across different locations were more variable (12-64%CV). In general, the protein concentrations in 2013 and 2014 show similar trends in variability. Some degree of variability in protein concentrations should be expected for both transgenic and endogenous plant-expressed proteins. In the case of GM crops, the potential variation in protein concentrations due to location effects is captured in the current model of multi-location field testing.
转基因（GM）作物经过大规模的多地点田间试验，以表征农艺学、组成和新表达蛋白的浓度[在此称为转基因蛋白（s）]。在 GM 作物的安全性评估中，考虑了不同植物组织和植物发育阶段的转基因蛋白浓度。参考或管家蛋白因其在细胞功能中的作用，预计在健康植物中保持相对稳定的表达模式。了解基因型、生长阶段和地点对内源性管家蛋白浓度的影响，可能有助于了解这些因素对 GM 作物中转基因蛋白浓度的贡献。在 2 年的多个田间地点种植的几种不同玉米杂种中，测量了三种内源性蛋白（肌动蛋白、延伸因子 1-α和甘油醛 3-磷酸脱氢酶）的浓度。从生长季节的三个发育阶段收集健康植物的叶片样本，并通过间接酶联免疫吸附测定（ELISA）定量每种蛋白的浓度。一般来说，与同一生长阶段和地点（2-26%CV）内相比，这三种内源性蛋白的浓度在杂种背景之间相对一致，而同一杂种和生长阶段在不同地点的蛋白浓度变化更大（12-64%CV）。一般来说，2013 年和 2014 年的蛋白质浓度变化趋势相似。对于转基因和内源植物表达的蛋白质，都应该预期存在一定程度的蛋白质浓度变化。在 GM 作物的情况下，由于位置效应导致的蛋白质浓度的潜在变化在当前的多地点田间试验模型中得到了捕捉。
