Herman Rod A, Fast Brandon J, Scherer Peter N, Brune Alyssa M, de Cerqueira Denise T, Schafer Barry W, Ekmay Ricardo D, Harrigan George G, Bradfisch Greg A
Dow AgroSciences LLC, Indianapolis, IN, USA.
Dow AgroSciencies Sementes e Biotecnologia Brasil LTDA, Cravinhos, SP, Brazil.
Plant Biotechnol J. 2017 Oct;15(10):1264-1272. doi: 10.1111/pbi.12713. Epub 2017 Apr 11.
The impact of crossing ('stacking') genetically modified (GM) events on maize-grain biochemical composition was compared with the impact of generating nonGM hybrids. The compositional similarity of seven GM stacks containing event DAS-Ø15Ø7-1, and their matched nonGM near-isogenic hybrids (iso-hybrids) was compared with the compositional similarity of concurrently grown nonGM hybrids and these same iso-hybrids. Scatter plots were used to visualize comparisons among hybrids and a coefficient of identity (per cent of variation explained by line of identity) was calculated to quantify the relationships within analyte profiles. The composition of GM breeding stacks was more similar to the composition of iso-hybrids than was the composition of nonGM hybrids. NonGM breeding more strongly influenced crop composition than did transgenesis or stacking of GM events. These findings call into question the value of uniquely requiring composition studies for GM crops, especially for breeding stacks composed of GM events previously found to be compositionally normal.
将转基因事件杂交（“堆叠”）对玉米籽粒生化成分的影响与培育非转基因杂交种的影响进行了比较。将含有事件DAS-Ø15Ø7-1的七个转基因堆叠与其匹配的非转基因近等基因杂交种（等基因杂交种）的成分相似性，与同期种植的非转基因杂交种和这些相同等基因杂交种的成分相似性进行了比较。散点图用于直观显示杂交种之间的比较，并计算一致性系数（由一致性线解释的变异百分比）以量化分析物谱内的关系。转基因育种堆叠的成分与其等基因杂交种的成分比非转基因杂交种的成分更相似。非转基因育种对作物成分的影响比转基因或转基因事件的堆叠更强。这些发现质疑了专门要求对转基因作物进行成分研究的价值，特别是对于由先前发现成分正常的转基因事件组成的育种堆叠。
