Chen Meijuan, Li Weiying, Teng Haibo, Hu Wenxin, Dong Zhiqiang, Zhang Dawei, Liu Tianyi, Zheng Quan
College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.
East Line Smart Water of China South-to-North Water Diversion Corporation Limited, Beijing 100071, China.
Antibiotics (Basel). 2024 Aug 5;13(8):734. doi: 10.3390/antibiotics13080734.
This study aimed to explore the response of archaeal communities and antibiotic-resistance genes (ARGs) to ciprofloxacin (CIP, 0.05-40 mg/L) and copper (Cu, 3 mg/L) combined pollution during stress- and post-effect periods in an activated sludge system. With the increase in the CIP concentration, the diversity of archaea decreased, but the richness increased under the stress of 10 mg/L CIP. Under stress and post effects, the change in unknown archaeal community structure was more significant than that of the known archaea. The relative abundance of unknown archaea was significantly reduced with the increase in CIP concentration. Meanwhile, there were certain archaea that belonged to abundant and rare taxa with different resistance and recovery characteristics. Among them, (49.15-83.66%), (0.11-0.45%), and (0.03-0.36%) were the typical resistant archaea to combined pollution. And the resistance of the abundant taxa to combined pollution was significantly higher than that of the rare taxa. Symbiotic and competitive relationships were observed between the known and the unknown archaea. The interactions of abundant known taxa were mainly symbiotic relationships. While the rare unknown taxa were mainly competitive relationships in the post-effect period. Rare archaea showed an important ecological niche under the stress-effect. Some archaea displayed positive correlation with ARGs and played important roles as potential hosts of ARGs during stress- and post-periods. , , and some rare unknown archaea also significantly co-occurred with a large number of ARGs. Overall, this study points out the importance of interactions among known and unknown archaeal communities and ARGs in a wastewater treatment system under the stress of antibiotics and heavy metal combined pollution.
本研究旨在探讨活性污泥系统中，古菌群落和抗生素抗性基因（ARGs）在应激期和后效应期对环丙沙星（CIP，0.05 - 40 mg/L）和铜（Cu，3 mg/L）联合污染的响应。随着CIP浓度的增加，古菌多样性降低，但在10 mg/L CIP的胁迫下，丰富度增加。在应激和后效应期间，未知古菌群落结构的变化比已知古菌更显著。随着CIP浓度的增加，未知古菌的相对丰度显著降低。同时，存在一些属于丰富和稀有类群的古菌，具有不同的抗性和恢复特征。其中，（49.15 - 83.66%）、（0.11 - 0.45%）和（0.03 - 0.36%）是联合污染的典型抗性古菌。丰富类群对联合污染的抗性显著高于稀有类群。已知古菌和未知古菌之间观察到共生和竞争关系。丰富的已知类群之间的相互作用主要是共生关系。而后效应期稀有未知类群主要是竞争关系。稀有古菌在应激效应下显示出重要的生态位。一些古菌与ARGs呈正相关，在应激期和后效应期作为ARGs的潜在宿主发挥重要作用。、、和一些稀有未知古菌也与大量ARGs显著共现。总体而言，本研究指出了在抗生素和重金属联合污染胁迫下，废水处理系统中已知和未知古菌群落与ARGs之间相互作用的重要性。
