Université Laval, Pavillon Alexandre Vachon, 1045, ave de la Médecine, Québec, QC G1V0A6, Canada; CRIUCPQ, 2725, Chemin Sainte-Foy, Québec, QC G1V 4G5, Canada.
CRIUCPQ, 2725, Chemin Sainte-Foy, Québec, QC G1V 4G5, Canada.
J Environ Sci (China). 2018 May;67:45-53. doi: 10.1016/j.jes.2017.07.015. Epub 2017 Jul 29.
Wastewater treatment center (WTC) workers may be vulnerable to diseases caused by viruses, such as the common cold, influenza and gastro-intestinal infections. Although there is a substantial body of literature characterizing the microbial community found in wastewater, only a few studies have characterized the viral component of WTC aerosols, despite the fact that most diseases affecting WTC workers are of viral origin and that some of these viruses are transmitted through the air. In this study, we evaluated in four WTCs the presence of 11 viral pathogens of particular concern in this milieu and used a metagenomic approach to characterize the total viral community in the air of one of those WTCs. The presence of viruses in aerosols in different locations of individual WTCs was evaluated and the results obtained with four commonly used air samplers were compared. We detected four of the eleven viruses tested, including human adenovirus (hAdV), rotavirus, hepatitis A virus (HAV) and Herpes Simplex virus type 1 (HSV1). The results of the metagenomic assay uncovered very few viral RNA sequences in WTC aerosols, however sequences from human DNA viruses were in much greater relative abundance.
污水处理中心（WTC）的工作人员可能容易感染病毒引起的疾病，如普通感冒、流感和肠胃感染。尽管有大量的文献描述了废水中存在的微生物群落，但只有少数研究描述了 WTC 气溶胶中的病毒成分，尽管大多数影响 WTC 工作人员的疾病都是由病毒引起的，而且其中一些病毒是通过空气传播的。在这项研究中，我们在四个 WTC 中评估了 11 种特别关注的病毒病原体的存在情况，并使用宏基因组方法对其中一个 WTC 的空气中的总病毒群落进行了特征描述。评估了不同位置的气溶胶中病毒的存在情况，并比较了四种常用空气采样器的结果。我们检测到了测试的 11 种病毒中的四种，包括人类腺病毒（hAdV）、轮状病毒、甲型肝炎病毒（HAV）和单纯疱疹病毒 1 型（HSV1）。宏基因组分析的结果显示，WTC 气溶胶中的病毒 RNA 序列很少，但人类 DNA 病毒的序列相对丰度更高。
