Protein Expression Facility, Manchester Institute of Biotechnology, Faculty of Life Sciences, 131 Princess Street, Manchester M1 7DN, UK.
Peak Proteins Ltd, Alderley Park, Cheshire SK10 4TG, UK.
Methods. 2018 Sep 1;147:40-49. doi: 10.1016/j.ymeth.2018.05.013. Epub 2018 May 18.
Purified recombinant proteins are key reagents in academic and industrial research. The ability to make these proteins quickly often relies on the availability of higher eukaryotic cell hosts such as insect and mammalian cells where there is a very wide range of post-translational modifications, protein folding and trafficking pathways. This enables the generation of many proteins that cannot be made in microbial hosts. In this article we outline some of the most commonly used methods to express recombinant proteins in insect and mammalian cells.
纯化的重组蛋白是学术和工业研究的关键试剂。快速生产这些蛋白质的能力通常依赖于高等真核细胞宿主的可用性，如昆虫和哺乳动物细胞，其中存在广泛的翻译后修饰、蛋白质折叠和运输途径。这使得能够生成许多在微生物宿主中无法制造的蛋白质。在本文中，我们概述了在昆虫和哺乳动物细胞中表达重组蛋白的一些最常用的方法。
