Gonçalves Andréia Souza, Oliveira Jéssica Petini, Oliveira Carolina Ferrari Piloni, Silva Tarcília Aparecida, Mendonça Elismauro Francisco, Wastowski Isabela Jubé, Batista Aline Carvalho
Department of Stomatology (Oral Pathology), Dental School, Federal University of Goiás, Goiânia, Brazil.
Department of Oral Surgery and Pathology, Dental School, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Hum Immunol. 2016 Sep;77(9):785-90. doi: 10.1016/j.humimm.2015.12.001. Epub 2015 Dec 23.
HLA-G, HLA-E and IL-10 are molecules which can provide tumor immunosuppression as well as the capacity of evasion to the immune system host. This study set out to evaluate HLA-G, HLA-E and IL-10 expression in lip squamous cell carcinoma (LSCC) and in a potentially malignant disorder (actinic cheilitis - AC), correlating the expression of these proteins with the degree of epithelial dysplasia. Immunohistochemistry was undertaken to identify HLA-G, HLA-E and IL-10 in samples from patients with LSCC (n=20), AC (n=30) and healthy lip mucosa (control) (n=10). A semiquantitative scoring system was used for analysis. Differences between the groups were evaluated using the Pearson Chi-Squared test. The percentage of LSCC samples showing high immunoreactivity (IRS>2) for HLA-G, HLA-E and IL-10 (neoplastic/epithelial cells) and HLA-E (stroma/connective tissue) was significantly higher that of the control (P<0.05). A tendency for a progressive increase in the proteins analyzed was observed from the control to AC and to LSCC. The degree of dysplasia in the AC samples was not significantly associated with the proteins evaluated (P>0.05). The high expression of HLA-G, HLA-E and IL-10 in AC and LSCC reflects the capacity that these pathologies have for evasion and progression.
HLA - G、HLA - E和IL - 10是能够提供肿瘤免疫抑制以及逃避宿主免疫系统能力的分子。本研究旨在评估HLA - G、HLA - E和IL - 10在唇鳞状细胞癌（LSCC）和潜在恶性疾病（光化性唇炎 - AC）中的表达，并将这些蛋白质的表达与上皮发育异常程度相关联。采用免疫组织化学方法在LSCC患者（n = 20）、AC患者（n = 30）和健康唇黏膜（对照）（n = 10）的样本中鉴定HLA - G、HLA - E和IL - 10。使用半定量评分系统进行分析。采用Pearson卡方检验评估组间差异。LSCC样本中HLA - G、HLA - E和IL - 10（肿瘤/上皮细胞）以及HLA - E（基质/结缔组织）显示高免疫反应性（IRS>2）的百分比显著高于对照组（P<0.05）。从对照组到AC组再到LSCC组，观察到所分析蛋白质有逐渐增加的趋势。AC样本中的发育异常程度与所评估的蛋白质无显著相关性（P>0.05）。AC和LSCC中HLA - G、HLA - E和IL - 10的高表达反映了这些病变的逃避和进展能力。
