Advanced Centre for Plant Virology, Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India.
Plant Cell Rep. 2018 Dec;37(12):1707-1712. doi: 10.1007/s00299-018-2297-2. Epub 2018 May 19.
This approach is quite promising to control plant viral diseases and create synthetic networks to better understand the structure/function relationship in RNA and proteins. Plant viruses are obligate intracellular parasites which causes enormous losses in crop yield worldwide. These viruses replicate into infected cells by highjacking host cellular machinery. Over the last two decades, diverse approaches such as conventional breeding, transgenic approach and gene silencing strategies have been used to control RNA viruses, but escaped due to high rate of mutation. Recently, a novel CRISPR enzyme, called Cas13a, has been used engineered to confer RNA viruses resistance in plants. Here, we summarize the recent breakthrough of CRISPR/Cas13a and its applications in RNA biology.
这种方法在控制植物病毒病和创建合成网络以更好地理解 RNA 和蛋白质的结构/功能关系方面非常有前景。植物病毒是专性细胞内寄生虫，在全球范围内导致作物产量巨大损失。这些病毒通过劫持宿主细胞机制在感染细胞中复制。在过去的二十年中，已经使用了多种方法，如常规育种、转基因方法和基因沉默策略来控制 RNA 病毒，但由于突变率高而逃脱。最近，一种新型的 CRISPR 酶，称为 Cas13a，已被用于工程改造以赋予植物对 RNA 病毒的抗性。在这里，我们总结了 CRISPR/Cas13a 的最新突破及其在 RNA 生物学中的应用。
