Xu Bin, Chen Bor-Yann, Huang Kuan-Chieh, Sun Qing-Jiang, Chi-Wei Lan John
State Key Laboratory of Bioelectronics, School of Biological Science & Medical Engineering, and Research Center for Learning Science, Southeast University, Nanjing 210096, PR China; Biorefinery and Bioprocess Engineering Laboratory, Department of Chemical Engineering and Materials Science, Yuan Ze University, Chungli, Taoyuan 320, Taiwan.
Department of Chemical and Materials Engineering, National I-Lan University, I-Lan 260, Taiwan.
J Biosci Bioeng. 2018 Sep;126(3):322-329. doi: 10.1016/j.jbiosc.2018.03.019. Epub 2018 May 18.
This first-attempt study tended to decipher synergistic interactions of model redox mediators (RMs) to echinenone production for electrochemically-steered fermentation (ESF). The findings indicated that supplement of RMs could significantly stimulate the production performance of fermentation (e.g., 36% for 4-aminophenol) which was parallel with stimulation of bioelectricity generation in microbial fuel cells (MFCs) as prior studies mentioned. Although redox mediators could usually enhance electron transport extracellular compartment, the mechanisms of bioelectricity generation in MFCs and echinenone production in ESF were very likely functioned in the extracellular and the intracellular compartment, respectively. In MFCs, electron transfer towards biofilm anode for bioelectricity generation must be taken place. However, for ESF echinenone accumulation was very likely occurred in the intracellular compartment, thus electron transfer was predominantly implemented in the intracellular, not the extracellular compartment.
这项首次尝试的研究旨在解析模型氧化还原介质（RM）对电化学调控发酵（ESF）中虾青素生产的协同相互作用。研究结果表明，添加RM可显著刺激发酵生产性能（例如，对4-氨基苯酚而言提高了36%），这与先前研究中微生物燃料电池（MFC）中生物电产生的刺激情况相似。尽管氧化还原介质通常可增强细胞外区域的电子传递，但MFC中生物电产生的机制与ESF中虾青素生产的机制很可能分别在细胞外和细胞内区域发挥作用。在MFC中，必须发生向生物膜阳极的电子转移以产生生物电。然而，对于ESF，虾青素的积累很可能发生在细胞内区域，因此电子转移主要在细胞内而非细胞外区域进行。
