Hu Weiyao, Li Zengping, Wang Xiaoye, Liu Wenbo, Huang Chaohao, Miao Weiguo, Cui Hongguang
College of Tropical Agriculture and Forestry, Hainan University, Haikou, 570228, Hainan, People's Republic of China.
Arch Virol. 2018 Sep;163(9):2579-2582. doi: 10.1007/s00705-018-3879-6. Epub 2018 May 21.
A macluravirus, tentatively named alpinia oxyphylla mosaic virus (AloMV), was identified in Alpinia oxyphylla, and its complete genomic sequence determined. The positively single-stranded RNA genome is comprised of 8213 nucleotides excluding the poly (A) tail, and contains one large open reading frame encoding a polyprotein of 2,626 amino acids. Blastp search showed that the polyprotein of AloMV shared 48%~68% aa sequence identities with other reported macluraviruses. Phylogenetic analysis based on the nucleotide sequence of the polyprotein showed that AloMV, together with all other macluraviruses, clustered into the same group most closely related to cardamom mosaic virus, sharing 66.3% nt and 68% aa sequence identities, respectively. These data above suggest that AloMV represents an isolate of a putative new member within the genus Macluravirus.
在益智中鉴定出一种暂定名为益智花叶病毒(AloMV)的马古鲁病毒,并测定了其完整的基因组序列。不含聚(A)尾的正向单链RNA基因组由8213个核苷酸组成,包含一个大的开放阅读框,编码一个由2626个氨基酸组成的多聚蛋白。Blastp搜索显示,AloMV的多聚蛋白与其他已报道的马古鲁病毒的氨基酸序列同一性为48%至68%。基于多聚蛋白核苷酸序列的系统发育分析表明,AloMV与所有其他马古鲁病毒一起聚为与小豆蔻花叶病毒关系最密切的同一组,分别共享66.3%的核苷酸和68%的氨基酸序列同一性。上述数据表明,AloMV代表马古鲁病毒属内一个假定新成员的分离株。