Yang Ke, Yan Haixia, Song Li, Jin Pengfei, Miao Weiguo, Cui Hongguang
College of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan, 570228, People's Republic of China.
College of Forestry, Agricultural University of Hebei, Baoding, Hebei, 071001, People's Republic of China.
Arch Virol. 2018 Sep;163(9):2583-2586. doi: 10.1007/s00705-018-3885-8. Epub 2018 May 22.
The complete genomic sequence of a telosma mosaic virus (TeMV) isolate (named PasFru), identified in passion fruit in China, was determined. The entire RNA genome of PasFru comprises 10,049 nucleotides (nt) excluding the poly(A) tail and encodes a polyprotein of 3,173 amino acids (aa), flanked by 5' and 3' untranslated regions (UTR) of 276 and 251 nt, respectively. Compared with the previous TeMV isolate Hanoi from Telosma cordata, the only documented isolate with the entire genome sequence annotated, PasFru had an extra 87 nt and 89 aa residues at the 3'-end of 5'UTR and the N-terminus of the P1 protein, respectively, which contributed to the genome size difference between PasFru and Hanoi (10,049 nt versus 9,689 nt). Pairwise sequence comparisons showed that PasFru shares 73.6% nt and 80.9% aa sequence identity with the Hanoi isolate at the whole-genome and polyprotein level, respectively, and these values are below the corresponding threshold values for species demarcation in the family Potyviridae. These data suggest that TeMV-PasFru should be classified as a new member of the genus Potyvirus.
在中国西番莲中鉴定出一种夜来香花叶病毒（TeMV）分离株（命名为PasFru），并测定了其完整基因组序列。PasFru的整个RNA基因组不包括多聚腺苷酸尾，由10,049个核苷酸（nt）组成，编码一个含3,173个氨基酸（aa）的多聚蛋白，两侧分别是276 nt和251 nt的5'和3'非翻译区（UTR）。与之前从夜来香中分离得到的TeMV分离株河内（Hanoi）相比，河内是唯一有完整基因组序列注释的记录分离株，PasFru在5'UTR的3'端和P1蛋白的N端分别额外有87 nt和89个aa残基，这导致了PasFru和河内之间的基因组大小差异（10,049 nt对9,689 nt）。成对序列比较表明，PasFru在全基因组和多聚蛋白水平上与河内分离株的核苷酸序列同一性分别为73.6%和氨基酸序列同一性为80.9%，这些值低于马铃薯Y病毒科物种划分的相应阈值。这些数据表明，TeMV-PasFru应被归类为马铃薯Y病毒属的一个新成员。
