Kalaeva E A, Artyukhov V G, Putintseva O V, Polyubez'eva A I
Eksp Klin Farmakol. 2016;79(9):12-17.
The spectral and oxygen-binding characteristics of human intracellular hemoglobin in the presence of nitroglycerin at concentrations of 5 ng/mL and 5 μg/mL have been studied. Short incubation (20 min) of erythrocytes with the drug led increasing hemoglobin affinity to oxygen and weakening of cooperative interactions in hemoprotein molecules. As a result, the amount of O(2) supplied to tissues in the process of gas exchange decreased by 23.96% (5 ng/mL) and 26.68% (5 μg/ml), p < 0.05. Incubation of cells for 24 h resulted in oxidation of the heme iron atom, accumulation of methemoglobin, and partial hemolysis. Nitroglycerin reduces the intensity of oxidative processes. However, no dependence of the degree of changes in the physical and chemical properties of hemoglobin on the concentration of nitroglycerin was found.
研究了在浓度为5 ng/mL和5 μg/mL的硝酸甘油存在下，人细胞内血红蛋白的光谱和氧结合特性。红细胞与该药物短时间孵育（20分钟）导致血红蛋白对氧的亲和力增加，血红蛋白分子中的协同相互作用减弱。结果，在气体交换过程中供应给组织的O(2)量减少了23.96%（5 ng/mL）和26.68%（5 μg/ml），p < 0.05。细胞孵育24小时导致血红素铁原子氧化、高铁血红蛋白积累和部分溶血。硝酸甘油降低了氧化过程的强度。然而，未发现血红蛋白物理和化学性质变化程度与硝酸甘油浓度之间的依赖性。
