School of Pharmaceutical Sciences, Xiamen University, Xiamen, 361102, China.
School of Life Sciences, Xiamen University, Xiamen, 361102, China.
Bioprocess Biosyst Eng. 2018 Aug;41(8):1213-1224. doi: 10.1007/s00449-018-1950-z. Epub 2018 May 22.
Microalgae are considered as attractive feedstocks for biofuel production nowadays because of their high lipid contents and easy cultivation. In the present study, two diatoms, Thalassiosira weissflogii and Chaetoceros muelleri, were cultured under various nutrient-limitation conditions to explore their comprehensive lipid accumulation profiles for further commercialization. In T. weissflogii, the highest neutral lipid accumulation and highest lipid productivity (14.28 mg L day) were both recorded under P-limitation. In C. muelleri, the highest lipid content (35.03% of dry cell weight), highest neutral lipid accumulation, and highest lipid productivity (29.07 mg L day) were all recorded under N-limitation. Besides, the predominant fatty acids of T. weissflogii and C. muelleri were myristic acid (C14:0), palmitic acid (C16:0), and palmitoleic acid (C16:1), with the amounts of 58.4-74.4 and 74.1-87.7% of the total fatty acids, respectively. Moreover, nutrient limitations led to a lower proportion of polyunsaturated fatty acids (PUFA) than that of saturated fatty acid (SFA) and monounsaturated fatty acid (MUFA) in both species. The ratios of (SFA + MUFA) to PUFA were from 1.65 to 3.01 in T. weissflogii, and up to 3.61 to 8.59 in C. muelleri. Our results suggested the feasibility of C. muelleri as biodiesel feedstock due to its more suitable fatty acid composition and higher lipid productivity compared to T. weissflogii.
微藻被认为是有吸引力的生物燃料生产原料，因为它们具有高脂质含量和易于培养。在本研究中，培养了两种硅藻，威氏海链藻和角毛藻，在各种营养限制条件下，以探索它们的综合脂质积累谱，以进一步实现商业化。在威氏海链藻中，在磷限制下，中性脂质积累最高和脂质生产力（14.28mgL 天）最高。在角毛藻中，最高的脂质含量（干细胞重量的 35.03%）、最高的中性脂质积累和最高的脂质生产力（29.07mgL 天）都是在氮限制下记录的。此外，威氏海链藻和角毛藻的主要脂肪酸是豆蔻酸（C14:0）、棕榈酸（C16:0）和棕榈油酸（C16:1），分别占总脂肪酸的 58.4-74.4%和 74.1-87.7%。此外，营养限制导致两种生物中多不饱和脂肪酸（PUFA）的比例低于饱和脂肪酸（SFA）和单不饱和脂肪酸（MUFA）。威氏海链藻中（SFA+MUFA）与 PUFA 的比例从 1.65 到 3.01，而在角毛藻中高达 3.61 到 8.59。我们的结果表明，由于角毛藻的脂肪酸组成更适合且脂质生产力高于威氏海链藻，因此它作为生物柴油原料是可行的。
