Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang 222005, China; State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China.
Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang 222005, China.
Bioresour Technol. 2019 Oct;289:121717. doi: 10.1016/j.biortech.2019.121717. Epub 2019 Jun 27.
To enhance biodiesel production and quality from a bloom-forming diatom Skeletonema costatum, a two-stage model, in which cells were cultured in nutrient replete conditions first and then transferred to nutrient limitation conditions, was explored. Compared to one-stage model, nutrient limitation in the second stage significantly increased lipid content in spite of decreasing growth; consequently, Si-limitation and N-Si-limitation respectively increased lipid productivity by 37.6% and 76.7% for 6 h induction, and 42.8% and 113.7% for 12 induction. Nutrient limitation enhanced the proportions of saturated fatty acids (SFA) and monounsaturated fatty acids (MUFA) but reduced polyunsaturated fatty acid (PUFA). Therefore, N-Si-limitation reduced iodine value by 33.7% and 45.6% but increased cetane number by 6.4% and 21.6% for 6 and 24 h induction, respectively. These findings indicate that the two-stage model with N-Si-limitation can enhance lipid productivity as well as biodiesel quality from diatoms.
为了提高形成赤潮的硅藻小环藻（Skeletonema costatum）的生物柴油产量和质量，本研究采用了两段式模型，即细胞先在营养充足的条件下培养，然后再转移到营养限制条件下。与一阶段模型相比，尽管生长受到抑制，但第二阶段的营养限制显著增加了油脂含量；因此，硅限制和氮硅限制分别使 6 小时诱导时的油脂生产力提高了 37.6%和 76.7%，12 小时诱导时提高了 42.8%和 113.7%。营养限制提高了饱和脂肪酸（SFA）和单不饱和脂肪酸（MUFA）的比例，但降低了多不饱和脂肪酸（PUFA）的比例。因此，氮硅限制使碘值分别降低了 33.7%和 45.6%，但使十六烷值分别提高了 6.4%和 21.6%，6 小时和 24 小时诱导时分别提高了 6.4%和 21.6%。这些发现表明，氮硅限制的两段式模型可以提高微藻油脂生产力和生物柴油质量。
