Department of Chemical and Materials Engineering, University of Kentucky, Lexington, KY, 40506-0046, USA.
Department of Biosystems and Agricultural Engineering, University of Kentucky, Lexington, KY, 40546-0276, USA.
Bioprocess Biosyst Eng. 2018 Sep;41(9):1283-1294. doi: 10.1007/s00449-018-1956-6. Epub 2018 May 22.
Process simulations of batch fermentations with in situ product separation traditionally decouple these interdependent steps by simulating a separate "steady state" continuous fermentation and separation units. In this study, an integrated batch fermentation and separation process was simulated for a model system of acetone-butanol-ethanol (ABE) fermentation with in situ gas stripping, such that the fermentation kinetics are linked in real-time to the gas stripping process. A time-dependent cell growth, substrate utilization, and product production is translated to an Aspen Plus batch reactor. This approach capitalizes on the phase equilibria calculations of Aspen Plus to predict the effect of stripping on the ABE fermentation kinetics. The product profiles of the integrated fermentation and separation are shown to be sensitive to gas flow rate, unlike separate steady state fermentation and separation simulations. This study demonstrates the importance of coupled fermentation and separation simulation approaches for the systematic analyses of unsteady state processes.
分批发酵的过程模拟传统上通过模拟独立的“稳态”连续发酵和分离单元来解耦这些相互依存的步骤。在这项研究中，模拟了一种带有原位气体汽提的丙酮丁醇乙醇（ABE）发酵的集成批式发酵和分离过程，使得发酵动力学实时与气体汽提过程相关联。时变细胞生长、基质利用和产物生成被转化为 Aspen Plus 间歇式反应器。这种方法利用了 Aspen Plus 的相平衡计算来预测汽提对 ABE 发酵动力学的影响。与独立的稳态发酵和分离模拟不同，集成发酵和分离的产物分布对气流速率敏感。本研究证明了耦合发酵和分离模拟方法对于非稳态过程的系统分析的重要性。
