Yan Zheng-Fei, Lin Pei, Li Chang-Tian, Kook MooChang, Yi Tae-Hoo
College of Life Science, Kyung Hee University Global Campus, 1732 Deokyoungdae-ro, Giheung-gu, Yongin-si, Gyeonggi-do, 17104, Republic of Korea.
Engineering Research Center of Edible and Medicinal Fungi, Ministry of Education, Jilin Agricultural University, Changchun, 130118, People's Republic of China.
Antonie Van Leeuwenhoek. 2018 Nov;111(11):2087-2094. doi: 10.1007/s10482-018-1101-1. Epub 2018 May 22.
A Gram-stain positive, aerobic, non-motile, endospore-forming and rod-shaped strain (THG-NT9) was isolated from a green tea sample. Growth occurred at 20-45 °C (optimum 28-35 °C), at pH 6.0-8.0 (optimum 7.0) and at 0-2.0% NaCl (optimum 0%). Based on 16S rRNA gene sequence analysis, the near phylogenetic neighbours of strain THG-NT9 were identified as Scopulibacillus daqui DSM 28236 (98.6%), Scopulibacillus darangshiensis DSM 19377 (97.4%), Pullulanibacillus pueri CGMCC 1.12777 (96.7%) and Pullulanibacillus camelliae CGMCC 1.15371 (96.3%). The DNA G + C content of strain THG-NT9 was determined to be 47.5 mol %. DNA-DNA hybridization values between strain THG-NT9 and S. daqui DSM 28236, S. darangshiensis DSM 19377, P. pueri CGMCC 1.12777, P. camelliae CGMCC 1.15371 and Pullulanibacillus naganoensis DSM 10191 were 41.3 ± 0.1 (39.4 ± 0.4% reciprocal analysis), 39.1 ± 0.1 (37.3 ± 0.1%), 21.4 ± 0.7 (20.1 ± 0.3%), 20.7 ± 0.1 (20.1 ± 0.4%) and 12.1 ± 0.2% (8.3 ± 0.2%). The polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and three unidentified lipids. The quinone was identified as MK-7. The major fatty acids were C ω7c, iso-C, iso-C, iso-C and anteiso-C. The cell wall type was determined to be A1γ peptidoglycan with meso-diaminopimelic acid as the diagnostic diamino acid plus alanine and glutamic acid and glucose as the cell wall sugar. On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics, and DNA-DNA hybridization data, strain THG-NT9 represents a novel species of the genus Scopulibacillus, for which the name Scopulibacillus cellulosilyticus sp. nov. is proposed. The type strain is THG-NT9 (= KCTC 33918 = CGMCC 1.16305).
从一份绿茶样品中分离出一株革兰氏染色阳性、需氧、不运动、产芽孢的杆状菌株（THG-NT9）。该菌株在20-45℃（最适温度28-35℃）、pH 6.0-8.0（最适pH 7.0）和0-2.0% NaCl（最适盐浓度0%）条件下生长。基于16S rRNA基因序列分析，菌株THG-NT9的近缘系统发育邻种被鉴定为达奎帚形芽孢杆菌DSM 28236（相似度98.6%）、大壤屎帚形芽孢杆菌DSM 19377（相似度97.4%）、普洱普鲁兰芽孢杆菌CGMCC 1.12777（相似度96.7%）和山茶普鲁兰芽孢杆菌CGMCC 1.15371（相似度96.3%）。菌株THG-NT9的DNA G+C含量测定为47.5 mol%。菌株THG-NT9与达奎帚形芽孢杆菌DSM 28236、大壤屎帚形芽孢杆菌DSM 19377、普洱普鲁兰芽孢杆菌CGMCC 1.12777、山茶普鲁兰芽孢杆菌CGMCC 1.15371以及长野普鲁兰芽孢杆菌DSM 10191之间的DNA-DNA杂交值分别为41.3±0.1（反向分析为39.4±0.4%）、39.1±0.1（37.3±0.1%）、21.4±0.7（20.1±0.3%）、20.7±0.1（20.1±0.4%）和12.1±0.2%（8.3±0.2%）。极性脂质被鉴定为双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺和三种未鉴定的脂质。醌类被鉴定为MK-7。主要脂肪酸为C ω7c、异-C、异-C、异-C和anteiso-C。细胞壁类型被确定为A1γ肽聚糖，以meso-二氨基庚二酸作为诊断性二氨基酸，加上丙氨酸、谷氨酸和葡萄糖作为细胞壁糖。基于系统发育分析、化学分类数据、生理特征和DNA-DNA杂交数据，菌株THG-NT9代表了帚形芽孢杆菌属的一个新物种，为此提出名为纤维素分解帚形芽孢杆菌新种（Scopulibacillus cellulosilyticus sp. nov.）。模式菌株为THG-NT9（=KCTC 33918 = CGMCC 1.16305）。
