Wang Yu-Shuai, Yan Zheng-Fei, Lin Pei, Gao Wei, Yi Tae-Hoo
Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University Global Campus, 1732 Deokyoungdae-ro, Giheung-gu, Yongin-Si, Gyeonggi-do, 17104, Republic of Korea.
Antonie Van Leeuwenhoek. 2018 Sep;111(9):1535-1542. doi: 10.1007/s10482-018-1041-9. Epub 2018 Feb 26.
A Gram-stain negative, aerobic, short rod-shaped, motile by flagella bacterial strain (THG-N2.35), was isolated from Pu'er tea. Growth occurred at 10-40 °C (optimum 28 °C), at pH 4-7 (optimum 7) and at 0-5% NaCl (optimum 1%). Based on 16S rRNA gene sequence analysis, the near phylogenetic neighbours of strain THG-N2.35 were identified as Paracoccus hibisci KACC 18632 (99.0%), Paracoccus tibetensis CGMCC 1.8925 (98.7%), Paracoccus beibuensis CGMCC 1.7295 (98.2%), Paracoccus aestuarii KCTC 22049 (98.2%), Paracoccus rhizosphaerae LMG 26205 (98.1%), Paracoccus zeaxanthinifaciens ATCC 21588 (97.1%), Paracoccus marcusii DSM 11574 (97.0%). Levels of similarity between strain THG-N2.35 and other Paracoccus species were lower than 97.0%. DNA-DNA hybridization values between strain THG-N2.35 and P. hibisci KACC 18632, P. tibetensis CGMCC 1.8925, P. beibuensis CGMCC 1.7295, P. aestuarii KCTC 22049, P. rhizosphaerae LMG 26205, P. zeaxanthinifaciens ATCC 21588, P.marcusii DSM 11574 were 47.5% (42.3%, reciprocal analysis), 36.1% (32.3%), 24.7% (22.1%), 19.2% (16.3%), 11.3% (8.8%), 11.1% (10.8%), 6.1% (5.8%), respectively. The DNA G+C content of strain THG-N2.35 was 62.3 mol%. The polar lipids were diphosphatidylglycerol, phosphatidyl-N-methylethanolamine, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine. The quinone was ubiquinone-10 (Q-10). The major fatty acids were C 3OH, C, C and C ω7ϲ. On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA-DNA hybridization data, strain THG-N2.35 represent a novel species of the genus Paracoccus, for which the name Paracoccus pueri sp. nov. is proposed. The type strain is THG-N2.35 (= KACC 18934 = CCTCC AB 2016177).
从普洱茶中分离出一株革兰氏染色阴性、需氧、短杆状、具鞭毛能运动的细菌菌株（THG-N2.35）。该菌株在10-40℃（最适温度28℃）、pH 4-7（最适pH 7）和0-5% NaCl（最适浓度1%）条件下生长。基于16S rRNA基因序列分析，菌株THG-N2.35的近缘系统发育邻种被鉴定为 Hibisci 副球菌KACC 18632（相似度99.0%）、西藏副球菌CGMCC 1.8925（98.7%）、北部湾副球菌CGMCC 1.7295（98.2%）、河口副球菌KCTC 22049（98.2%）、根际副球菌LMG 26205（98.1%）、产玉米黄质副球菌ATCC 21588（97.1%）、马库斯副球菌DSM 11574（97.0%）。菌株THG-N2.35与其他副球菌属物种的相似度低于97.0%。菌株THG-N2.35与 Hibisci 副球菌KACC 18632、西藏副球菌CGMCC 1.8925、北部湾副球菌CGMCC 1.7295、河口副球菌KCTC 22049、根际副球菌LMG 26205、产玉米黄质副球菌ATCC 21588、马库斯副球菌DSM 11574的DNA-DNA杂交值分别为47.5%（反向分析为42.3%）、36.1%（32.3%）、24.7%（22.1%）、19.2%（16.3%）、11.3%（8.8%）、11.1%（10.8%）、6.1%（5.8%）。菌株THG-N2.35的DNA G+C含量为62.3 mol%。极性脂为双磷脂酰甘油、磷脂酰-N-甲基乙醇胺、磷脂酰乙醇胺、磷脂酰甘油和磷脂酰胆碱。醌为泛醌-10（Q-10）。主要脂肪酸为C 3OH、C、C和C ω7ϲ。基于系统发育分析、化学分类数据、生理特征和DNA-DNA杂交数据，菌株THG-N2.35代表副球菌属的一个新物种，提议将其命名为普洱茶副球菌（Paracoccus pueri sp. nov.）。模式菌株为THG-N2.35（=KACC 18934 = CCTCC AB 2016177）。
