Sultanpuram Vishnuvardhan Reddy, Mothe Thirumala, Mohammed Farooq
Microbial Ecology Lab, Department of Biochemistry, Mahatma Gandhi University, Anneparthy, Yellareddygudem (PO), Nalgonda, 508254, India,
Arch Microbiol. 2015 May;197(4):553-60. doi: 10.1007/s00203-015-1081-8. Epub 2015 Feb 1.
A Gram stain-positive, rod-shaped, non-motile, orange-pigmented and non-endospore-forming novel bacterial strain 10nlg(T) was isolated from Lonar soda lake in India. Based on the 16S rRNA gene sequence analysis, it was belonged to the genus Salisediminibacterium and was most closely related to Salisediminibacterium halotolerans CGMCC 1.7654(T) (99.9 %), Bacillus locisalis CGMCC 1.6286(T) (99.1 %) and other members in the Bacillaceae (<93.6 %). Further, DNA-DNA hybridization results demonstrated that strain 10nlg(T) was distantly (<70 %) related to S. halotolerans halo-2(T) (59.6 %) and B. locisalis CGMCC 1.6286(T) (53.2 %). Strain 10nlg(T) was catalase positive and oxidase negative. The cell wall of the strain 10nlg(T) contains meso-diaminopimelic acid as the diagnostic amino acid. Polar lipids include diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, five phospholipids and two unknown lipids. The predominant isoprenoid quinone was MK-7. Anteiso-C15:0 (32.6 %) was the predominant fatty acid, and significant proportions of iso-C15:0 (9.3 %), anteiso-C17:0 (8.3 %), C16:0 (4.3 %) were also detected in the strain 10nlg(T). The DNA G+C content of the strain 10nlg(T) was 45.6 mol %. The results of phylogenetic, physiological and biochemical tests allowed a distinct differentiation of strain 10nlg(T) not only from S. halotolerans halo-2(T), the only member of the genus Salisediminibacterium, but also from the related Bacillaceae members. Strain 10nlg(T) represents a novel species of the genus Salisediminibacterium for which the name Salisediminibacterium haloalkalitolerans sp. nov. is proposed. The type strain is 10nlg(T) (=KCTC 33414(T) = CGMCC 1.12818(T)). B. locisalis CGMCC 1.6286(T) also represents a member of the genus Salisediminibacterium for which the name Salisediminibacterium locisalis CG1(T) is proposed. The type strain is CG1(T) = (CCM 7370(T) = CECT 7152(T) = CGMCC 1.6286(T) = DSM 18085(T)). In accordance with the new polar lipid data collected in this study, emended descriptions of the genus Salisediminibacterium and the species S. halotolerans halo-2(T) are also provided.
从印度洛纳尔盐湖分离出一株革兰氏染色阳性、杆状、无运动性、产橙色色素且不形成芽孢的新型细菌菌株10nlg(T)。基于16S rRNA基因序列分析，它属于盐栖盐杆菌属，与耐盐盐栖盐杆菌CGMCC 1.7654(T)（99.9%）、芽孢杆菌CGMCC 1.6286(T)（99.1%）以及芽孢杆菌科的其他成员（<93.6%）关系最为密切。此外，DNA-DNA杂交结果表明，菌株10nlg(T)与耐盐盐杆菌halo-2(T)（59.6%）和芽孢杆菌CGMCC 1.6286(T)（53.2%）的亲缘关系较远（<70%）。菌株10nlg(T)过氧化氢酶阳性，氧化酶阴性。菌株10nlg(T)的细胞壁含有内消旋二氨基庚二酸作为诊断性氨基酸。极性脂质包括双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、五种磷脂和两种未知脂质。主要的类异戊二烯醌是MK-7。anteiso-C15:0（32.6%）是主要脂肪酸，在菌株10nlg(T)中还检测到相当比例的iso-C15:0（9.3%）、anteiso-C17:0（8.3%）、C16:0（4.3%）。菌株10nlg(T)的DNA G+C含量为45.6 mol%。系统发育、生理和生化测试结果不仅使菌株10nlg(T)与盐栖盐杆菌属的唯一成员耐盐盐杆菌halo-2(T)区分开来，也与相关的芽孢杆菌科成员区分开来。菌株10nlg(T)代表盐栖盐杆菌属的一个新物种，为此提出盐栖耐盐碱盐杆菌新种（Salisediminibacterium haloalkalitolerans sp. nov.）。模式菌株为10nlg(T)（=KCTC 33414(T) = CGMCC 1.12818(T)）。芽孢杆菌CGMCC 1.6286(T)也代表盐栖盐杆菌属的一个成员，为此提出盐栖盐湖芽孢杆菌CG1(T)（Salisediminibacterium locisalis CG1(T)）。模式菌株为CG1(T) =（CCM 7370(T) = CECT 7152(T) = CGMCC 1.6286(T) = DSM 18085(T)）。根据本研究收集的新的极性脂质数据，还提供了盐栖盐杆菌属和耐盐盐杆菌halo-2(T)种的修订描述。
