银纳米颗粒对原绿球藻属微藻的活性。
The activity of silver nanoparticles against microalgae of the Prototheca genus.
机构信息
Department of Applied Microbiology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Warsaw, I. Miecznikowa 1, 02-096, Poland.
Laboratory of Electron & Confocal Microscopy, Faculty of Biology, University of Warsaw, I. Miecznikowa 1, 02-096, Warsaw, Poland.
出版信息
Nanomedicine (Lond). 2018 May;13(9):1025-1036. doi: 10.2217/nnm-2017-0370. Epub 2018 May 23.
AIM
To investigate the in vitro activity of silver NPs (AgNPs) against pathogenic microalgae of the Prototheca genus.
MATERIALS & METHODS: The antialgal potential of AgNPs against Prototheca species of both clinical and environmental origin was assessed from minimum inhibitory (algistatic) and algicidal concentrations. The in vitro cytotoxicity of AgNPs against bovine mammary epithelial cell line was evaluated by means of the standard MTT assay.
RESULTS
AgNPs showed a strong killing activity toward Prototheca algae, as the minimal algicidal concentration (MAC) values matched perfectly the corresponding minimum inhibitory concentration (MIC) values for all species (MAC = MIC, 1-4 mg/l), except P. stagnora (MIC > 8 mg/l). The concentrations inhibitory to pathogenic Prototheca spp. (MIC, 1-4 mg/l) were below the concentrations at which any toxicity in epithelial cells could be observed (CC > 6 mg/l).
CONCLUSION
The study emphasizes the potential of AgNPs as a new therapeutic tool for the management of Prototheca infections.
目的
研究银纳米粒子（AgNPs）对原绿球藻属致病微藻的体外活性。
材料与方法
从最小抑菌（抑藻）浓度和杀藻浓度评估 AgNPs 对临床和环境来源的原绿球藻属种的抗藻潜力。采用标准 MTT 测定法评估 AgNPs 对牛乳腺上皮细胞系的体外细胞毒性。
结果
AgNPs 对原绿球藻表现出很强的杀伤活性，因为所有物种的最小杀藻浓度（MAC）值与相应的最小抑菌浓度（MIC）值完全吻合（MAC=MIC，1-4mg/l），除了 P. stagnora（MIC＞8mg/l）。对致病性原绿球藻 spp.（MIC，1-4mg/l）有抑制作用的浓度低于在观察到上皮细胞任何毒性的浓度（CC＞6mg/l）。
结论
该研究强调了 AgNPs 作为治疗原绿球藻感染的新治疗工具的潜力。
Zotero 插件