Bartosińska Joanna, Purkot Joanna, Kowal Małgorzata, Michalak-Stoma Anna, Krasowska Dorota, Chodorowska Grażyna, Giannopoulos Krzysztof
Department of Dermatology, Venereology and Pediatric Dermatology, Medical University of Lublin, Poland.
Department of Experimental Hematooncology, Medical University of Lublin, Poland.
Adv Clin Exp Med. 2018 Jun;27(6):721-725. doi: 10.17219/acem/78020.
Psoriasis is a chronic autoinflammatory disease whose underlying molecular mechanisms remain unclear. The disease is mediated by the cells and molecules of both the innate and adaptive immune systems. Some T cell surface molecules, including neuropilin-1 (NRP1), programmed death 1 (PD-1) and the human leukocyte antigen G (HLA-G), are known to play a role in the maintenance of immune tolerance.
The aim of this study was to investigate HLA-G, NRP1 and programmed cell death gene (PDCD1) mRNA expression in psoriatic patients.
The study included 72 psoriatic patients and 35 healthy individuals. Twentyone patients (29.17%) suffered from concomitant psoriatic arthritis. The mRNA expression of HLA-G, NRP1, and PDCD1 were determined using quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). The severity of skin lesions was assessed by means of the Psoriasis Area and Severity Index (PASI), Body Surface Area (BSA), the Patient Global Assessment (PGA), and the Dermatology Life Quality Index (DLQI).
The median value of the PASI was 11.5, and of BSA was 15.8%. The expressions of NRP1 and PDCD1, but not HLA-G, were significantly lower in psoriatic patients in comparison with the control group. The expression of HLA-G, NRP1 and PDCD1 were not significantly different in the psoriatic arthritis and psoriasis vulgaris patients.
The results of this study suggest that the molecular markers of immune tolerance, i.e., HLA-G, NRP1, and PD-1, may be involved in the immune response in psoriatic patients.
银屑病是一种慢性自身炎症性疾病，其潜在分子机制尚不清楚。该疾病由先天性和适应性免疫系统的细胞和分子介导。已知一些T细胞表面分子，包括神经纤毛蛋白-1（NRP1）、程序性死亡1（PD-1）和人类白细胞抗原G（HLA-G），在维持免疫耐受中发挥作用。
本研究旨在调查银屑病患者中HLA-G、NRP1和程序性细胞死亡基因（PDCD1）的mRNA表达。
本研究纳入72例银屑病患者和35名健康个体。21例患者（29.17%）患有伴发的银屑病关节炎。使用定量实时逆转录聚合酶链反应（qRT-PCR）测定HLA-G、NRP1和PDCD1的mRNA表达。通过银屑病面积和严重程度指数（PASI）、体表面积（BSA）、患者整体评估（PGA）和皮肤病生活质量指数（DLQI）评估皮肤病变的严重程度。
PASI的中位数为11.5，BSA的中位数为15.8%。与对照组相比，银屑病患者中NRP1和PDCD1的表达显著降低，但HLA-G的表达无显著差异。银屑病关节炎患者和寻常型银屑病患者中HLA-G、NRP1和PDCD1的表达无显著差异。
本研究结果表明，免疫耐受的分子标志物，即HLA-G、NRP1和PD-1，可能参与银屑病患者的免疫反应。
