Medical University of Lodz, Łódź, Poland (Department of Dermatology and Venereology).
Medical University of Lodz, Łódź, Poland (Department of Biomedicine and Genetics).
Int J Occup Med Environ Health. 2020 Sep 17;33(5):635-647. doi: 10.13075/ijomeh.1896.01513. Epub 2020 Jul 22.
As a chronic, recurrent, immunologically mediated systemic disease and a common cause of dermatological problems, psoriasis is often a subject of scientific research. Skin changes located on the hands can cause difficulties and limitations in the performance of professional activities, especially manual ones. The main role in pathogenesis is played by immunological factors - improper functioning of the components of the immune system, among others, T lymphocytes and cytokines like interleukin-12 (IL-12), interleukin-22 (IL-22) and interferon gamma (IFN-γ).
The obtained tissue and blood were destined for RNA isolation. The RNA was then subjected to a reverse transcription reaction. The relative gene expression level was evaluated by the real-time polymerase chain reaction for , and genes, and presented as the relative quantification (RQ) value, relative to the reference gene . In addition, a correlation analysis of the expression level of selected genes with the clinical course of the disease, as assessed by the (PASI), the (BSA) and the (DLQI) scores was performed.
Statistical analysis confirmed a significant increase in RQ values for, and in the group of psoriatic patients vs. the control group. A positive correlation was also found between BSA and PASI and RQ for the gene.
Increased expression levels of , and genes in psoriatic skin confirm that selected cytokines play an important role in the initiation and sustenance of psoriasis. Int J Occup Med Environ Health. 2020;33(5):635-47.
银屑病是一种慢性、复发性、免疫介导的系统性疾病，也是皮肤科常见问题之一，因此经常成为科学研究的对象。位于手部的皮肤变化可能会导致患者在进行专业活动（尤其是手工活动）时遇到困难和限制。发病机制的主要作用因素是免疫因素——免疫系统的组成部分功能不当，其中包括 T 淋巴细胞和细胞因子，如白细胞介素-12（IL-12）、白细胞介素-22（IL-22）和干扰素γ（IFN-γ）。
获得的组织和血液用于 RNA 分离。然后将 RNA 进行逆转录反应。通过实时聚合酶链反应评估基因和的相对基因表达水平，并以相对于参考基因的相对定量（RQ）值表示。此外，还对所选基因的表达水平与疾病临床病程（通过 PASI、BSA 和 DLQI 评分评估）之间的相关性进行了分析。
统计分析证实，与对照组相比，银屑病患者组的 RQ 值、和显著增加。还发现 BSA 和 PASI 与基因的 RQ 值呈正相关。
银屑病皮肤中基因和的表达水平升高，证实了所选细胞因子在银屑病的发生和维持中起着重要作用。国际职业医学与环境卫生杂志。2020 年；33（5）：635-47。
