Department of Chemistry and Green-Nano Materials Research Center, Kyungpook National University, 80 Daehak-ro, Buk-gu, Daegu, 41566, Korea.
Department of Chemistry and Center for Cell-Encapsulation Research, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon, 34141, Korea.
Chemistry. 2018 Oct 22;24(59):15725-15743. doi: 10.1002/chem.201801710. Epub 2018 Jul 30.
Stem cells opened great opportunity to overcome diseases that conventional therapy had only limited success. Use of scaffolds made from biomaterials not only helps handling of stem cells for delivery or transplantation but also supports enhanced cell survival. Likewise, cell encapsulation can provide stability for living animal cells even in a state of separateness. Although various chemical reactions were tried to encapsulate stolid microbial cells such as yeasts, a culture environment for the growth of animal cells allows only highly biocompatible reactions. Therefore, the animal cells were mostly encapsulated in hydrogels, which resulted in enhanced cell survival. Interestingly, major findings of chemistry on biological interfaces demonstrate that cell encapsulation in hydrogels have a further a competence for modulating cell characteristics that can go beyond just enhancing the cell survival. In this review, we present a comprehensive overview on the chemical reactions applied to hydrogel-based cell encapsulation and their effects on the characteristics and behavior of living animal cells.
干细胞为克服传统疗法疗效有限的疾病提供了巨大的机会。使用生物材料制成的支架不仅有助于干细胞的处理、输送或移植，还有助于提高细胞存活率。同样，细胞包封即使在分离状态下也可以为活的动物细胞提供稳定性。尽管尝试了各种化学反应来包封固态微生物细胞（如酵母），但动物细胞的培养环境只允许高度生物相容的反应。因此，动物细胞大多被包封在水凝胶中，这导致了细胞存活率的提高。有趣的是，生物学界面化学的主要发现表明，水凝胶中的细胞包封在调节细胞特性方面具有进一步的能力，其效果超出了增强细胞存活率。在这篇综述中，我们全面介绍了应用于基于水凝胶的细胞包封的化学反应及其对活的动物细胞特性和行为的影响。
