Liu Yumei, Dong Ruiqi, Zhang Chunyan, Yang Yuxiang, Xu Yaolu, Wang Haojie, Zhang Mengyu, Zhu Jiamin, Wang Yuqin, Sun Yanhong, Zhang Ziqiang
College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan 471023, P.R. China.
Engineering Research Center for Mutton Sheep Breeding of Henan Province, Luoyang, Henan 471023, P.R. China.
Exp Ther Med. 2020 Jan;19(1):223-231. doi: 10.3892/etm.2019.8203. Epub 2019 Nov 15.
Peripheral nerve injury (PNI) is a common condition, often resulting from physical nerve injury and trauma. Successful repair of the peripheral nerve is dependent on the regenerative activity of Schwann cells (SCs). Application of SC-like adipose-derived mesenchymal stem cells (ADSCs) may be a suitable cell-based therapy for PNI. In the present study, nerve leachate derived from the rat sciatic nerve was used to induce the differentiation of ADSCs. These cells were placed in an acellular biological scaffold, which was then grafted to a rat sciatic nerve to bridge a 1-cm gap. Sprague-Dawley rats were divided into four groups: Scaffold only, untreated ADSCs + scaffold, nerve leachate-treated ADSCs + scaffold and autograft. Two-months post-transplant, the structure and function of the regenerated nerves and the recovery of the innervated muscles was analyzed. After transplant, there was a significant increase in the average area (15.86%; P<0.05), density (23.13%; P<0.05) and thickness (43.24%; P<0.05) of regenerated nerve fibers in the nerve leachate-treated ADSCs + scaffold group compared with the untreated ADSCs + scaffold group. The nerve conduction velocity in the nerve leachate-treated ADSCs + scaffold and autograft groups was superior to that in the other groups. In the nerve leachate-treated ADSCs + scaffold group, the cross-sectional area of the gastrocnemius increased by 39.28% (P<0.05) and the cross-sectional area of collagen fibers decreased by 29.87% (P<0.05) compared with the ADSCs + scaffold group. Moreover, the therapeutic effect of nerve leachate-treated ADSCs + scaffold on PNI was similar to that of an autograft. These results suggest that nerve leachate-treated ADSCs may promote the repair of PNI.
周围神经损伤（PNI）是一种常见病症，通常由物理性神经损伤和创伤引起。周围神经的成功修复取决于施万细胞（SCs）的再生活性。应用类似施万细胞的脂肪来源间充质干细胞（ADSCs）可能是一种适用于PNI的基于细胞的治疗方法。在本研究中，使用源自大鼠坐骨神经的神经浸出液诱导ADSCs分化。将这些细胞置于无细胞生物支架中，然后将其移植到大鼠坐骨神经上以桥接1厘米的间隙。将Sprague-Dawley大鼠分为四组：仅支架组、未处理的ADSCs +支架组、神经浸出液处理的ADSCs +支架组和自体移植组。移植后两个月，分析再生神经的结构和功能以及受神经支配肌肉的恢复情况。移植后，与未处理的ADSCs +支架组相比，神经浸出液处理的ADSCs +支架组再生神经纤维的平均面积（增加15.86%；P<0.05）、密度（增加23.13%；P<0.05）和厚度（增加43.24%；P<0.05）均有显著增加。神经浸出液处理的ADSCs +支架组和自体移植组的神经传导速度优于其他组。与ADSCs +支架组相比，神经浸出液处理的ADSCs +支架组中腓肠肌的横截面积增加了39.28%（P<0.05），胶原纤维的横截面积减少了29.87%（P<0.05）。此外，神经浸出液处理的ADSCs +支架组对PNI的治疗效果与自体移植相似。这些结果表明，神经浸出液处理的ADSCs可能促进PNI的修复。
