Huang Fang, Liu Jiaxiong, Liu Yiliu
South China Advanced Institute for Soft Matter Science and Technology, School of Emergent Soft Matter, South China University of Technology Guangzhou 510640 China
Guangdong Provincial Key Laboratory of Functional and Intelligent Hybrid Materials and Devices, South China University of Technology Guangzhou 510640 China.
Chem Sci. 2022 Jul 6;13(30):8885-8894. doi: 10.1039/d2sc02797f. eCollection 2022 Aug 4.
Supramolecular polymer chemistry, which closely integrates noncovalent interactions with polymeric structures, is a promising toolbox for living cell engineering. Here, we report our recent progress in exploring the applications of cucurbit[7]uril (CB[7])-based supramolecular polymer chemistry for engineering living cells. First, a modular polymer-analogous approach was established to prepare multifunctional polymers that contain CB[7]-based supramolecular recognition motifs. The supramolecular polymeric systems were successfully applied to cell surface engineering and subcellular organelle manipulation. By anchoring polymers on the cell membranes, cell-cell interactions were established by CB[7]-based host-guest recognition, which further facilitated heterogeneous cell fusion. In addition to cell surface engineering, placing the multifunctional polymers on specific subcellular organelles, including the mitochondria and endoplasmic reticulum, has led to enhanced physical contact between subcellular organelles. It is highly anticipated that the CB[7]-based supramolecular polymer chemistry will provide a new strategy for living cell engineering to advance the development of cell-based therapeutic materials.
超分子聚合物化学将非共价相互作用与聚合物结构紧密结合，是用于活细胞工程的一个很有前景的工具箱。在此，我们报告我们在探索基于葫芦[7]脲（CB[7]）的超分子聚合物化学在活细胞工程中的应用方面的最新进展。首先，建立了一种模块化的聚合物类似方法来制备含有基于CB[7]的超分子识别基序的多功能聚合物。超分子聚合物体系成功应用于细胞表面工程和亚细胞器操纵。通过将聚合物锚定在细胞膜上，基于CB[7]的主客体识别建立了细胞间相互作用，这进一步促进了异质细胞融合。除了细胞表面工程，将多功能聚合物放置在特定的亚细胞器上，包括线粒体和内质网，导致亚细胞器之间的物理接触增强。人们高度期待基于CB[7]的超分子聚合物化学将为活细胞工程提供一种新策略，以推动基于细胞的治疗材料的发展。
