Sports Sciences Department, Faculty of Literature & Human Sciences, Lorestan University, Khorramabad, Iran.
Medical Physiology Department, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.
J Diabetes Investig. 2019 Jan;10(1):43-50. doi: 10.1111/jdi.12868. Epub 2018 Jun 14.
AIMS/INTRODUCTION: To investigate the effect of endurance training on hippocampus DJ-1 and cannabinoid receptor type 2 (CB ) protein and blood glucose concentration in diabetic rats.
A total of 32 rats were randomly divided into diabetic (D), diabetic and exercise (DE), exercise (E) and control (C) groups. The endurance training was carried out five times per week for 6 weeks. The hippocampus DJ-1 and CB were measured using an enzyme-linked immunosorbent assay method.
The level of DJ-1 in the D group was significantly higher than the other groups (P ≤ 0.01). However, the level of DJ-1 was not significantly different between the C, E and DE groups. In addition, the level of CB was significantly lower in the D group compared with the other groups (P ≤ 0.01). Blood glucose was significantly higher in the D group compared with the DE group (P ≤ 0.05). Furthermore, a significant positive correlation between the level of DJ-1 and blood glucose was observed (r = 0.67, P ≤ 0.001). There was also a significant inverse correlation between the level of CB and blood glucose (r = -0.77, P ≤ 0.001).
The results of this study suggest that the level of DJ-1 and CB might change in response to diabetes, and regular aerobic exercise could mediate the effect of DJ-1 and CB on diabetes-induced neurodegenerative diseases.
目的/引言：研究耐力训练对糖尿病大鼠海马 DJ-1 和大麻素受体 2（CB）蛋白及血糖浓度的影响。
将 32 只大鼠随机分为糖尿病（D）组、糖尿病合并运动（DE）组、运动（E）组和对照组（C）组。每周进行 5 次耐力训练，共 6 周。采用酶联免疫吸附法检测海马 DJ-1 和 CB。
D 组 DJ-1 水平明显高于其他组（P≤0.01），而 C、E 和 DE 组之间 DJ-1 水平无明显差异。此外，D 组 CB 水平明显低于其他组（P≤0.01），D 组血糖明显高于 DE 组（P≤0.05）。DJ-1 水平与血糖呈显著正相关（r=0.67，P≤0.001），CB 水平与血糖呈显著负相关（r=-0.77，P≤0.001）。
本研究结果表明，DJ-1 和 CB 的水平可能会因糖尿病而发生变化，而规律的有氧运动可能会调节 DJ-1 和 CB 对糖尿病引起的神经退行性疾病的影响。
