Key laboratory of the vegetable postharvest treatment of Ministry of Agriculture, Beijing Key Laboratory of Fruits and Vegetable Storage and Processing, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China) of Ministry of Agriculture, Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China; Boyce Thompson Institute for Plant Research, Cornell University Campus, Ithaca, NY 14853, USA.
Beijing Academy of Forestry and Pomology Sciences, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100093, China.
Gene. 2018 Sep 10;671:142-151. doi: 10.1016/j.gene.2018.05.064. Epub 2018 May 21.
Cowpea is an important horticultural crop in tropical and subtropical areas of Asia, Africa, and Latin America, as well as parts of southern Europe and Central and South America. Chilling injury is a common physiological hazard of cowpea in cold chain logistics which reduce the cowpea pods nutritional quality and product value. However, the molecular mechanism involved in chilling injury remains unclear in cowpea pods. RNA-Seq and sRNA-Seq technologies were employed to decipher the miRNAs and mRNAs expression profiles and their regulatory networks in cowpea pods involved in chilling stress. Differentially expressed miRNAs and mRNA profiles were obtained based on cluster analysis, miRNAs and target genes were found to show coherent relationships in the regulatory networks of chilling injury. Furthermore, we found that numerous miRNAs and nat-siRNAs' targets were predicted to be key enzymes involved in the redox reactions such as POD, CAT, AO and LOX, energy metabolism such as ATPase, FAD and NAD related enzymes and different transcription factors such as WRKY, bHLH, MYB, ERF and NAC which play important roles in chilling injury.
菜豆是亚洲、非洲和拉丁美洲热带和亚热带地区以及欧洲南部和中美洲和南美洲部分地区的重要园艺作物。冷害是菜豆冷链物流中的一种常见生理危害，会降低菜豆花荚的营养品质和产品价值。然而，菜豆花荚冷害的分子机制尚不清楚。本研究采用 RNA-Seq 和 sRNA-Seq 技术，解析了菜豆花荚在冷胁迫下涉及的 miRNA 和 mRNAs 表达谱及其调控网络。基于聚类分析获得了差异表达的 miRNA 和 mRNA 谱，发现调控网络中 miRNA 和靶基因之间存在一致性关系。此外，我们发现许多 miRNA 和 nat-siRNA 的靶标预测为与氧化还原反应（如 POD、CAT、AO 和 LOX）、能量代谢（如 ATPase、FAD 和 NAD 相关酶）和不同转录因子（如 WRKY、bHLH、MYB、ERF 和 NAC）相关的关键酶，这些酶在冷害中发挥重要作用。
