School of Life Sciences, Zhengzhou University, Zhengzhou 450001, China.
School of Life Sciences, Zhengzhou University, Zhengzhou 450001, China.
Int J Biol Macromol. 2018 Oct 1;117:256-263. doi: 10.1016/j.ijbiomac.2018.05.134. Epub 2018 May 21.
Dictyopteris divaricata is a kind of important brown algae with many biological activities. It has been receiving more and more attention, yet there are rarely studies done on its polysaccharides. In this study, the optimum extraction and biological activity of seaweed polysaccharides from Dictyopteris divaricata (DDSP) were investigated. Response surface methodology (RSM), based on a three-level, three-variable Box-Behnken design (BBD), was employed to obtain the best possible combinations for maximum polysaccharides yield. The optimum extraction conditions were as follows: liquid-solid ratio of 110 mL/g, extraction time of 6 h and extraction temperature of 100 °C. Under these conditions, the experimental yield was 3.05%, which was in close agreement with the predicted value of 3.15%. The average molecular weight of DDSP was 58.05 kDa. Gas chromatograph (GC) results showed that DDSP was composed of fucose, xylose, mannose, glucose and galactose with the corresponding molar ratio of 4.45:2.74:1.00:2.94:1.35. Biological activity showed that DDSP exhibited strong antioxidant activity in vitro and possessed the potential on stimulating immune response of RAW264.7 cells. So DDSP can be used as a natural ingredient in functional foods.
叉羽藻是一种具有多种生物活性的重要褐藻。它越来越受到关注，但对其多糖的研究很少。本研究对叉羽藻多糖（DDSP）的最佳提取条件和生物活性进行了研究。基于三水平三变量 Box-Behnken 设计（BBD）的响应面法（RSM）用于获得多糖产率最大的最佳组合。最佳提取条件为：液固比 110 mL/g、提取时间 6 h、提取温度 100 °C。在此条件下，实验产率为 3.05%，与预测值 3.15%非常接近。DDSP 的平均分子量为 58.05 kDa。气相色谱（GC）结果表明，DDSP 由岩藻糖、木糖、甘露糖、葡萄糖和半乳糖组成，摩尔比为 4.45:2.74:1.00:2.94:1.35。生物活性表明，DDSP 具有较强的体外抗氧化活性，并具有刺激 RAW264.7 细胞免疫应答的潜力。因此，DDSP 可用作功能性食品中的天然成分。
