College of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, Guangxi, China; State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Nanning 530004, Guangxi, China.
College of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, Guangxi, China; State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Nanning 530004, Guangxi, China.
Int J Biol Macromol. 2018 Oct 1;117:108-115. doi: 10.1016/j.ijbiomac.2018.05.147. Epub 2018 May 22.
Millettia dielsiana is a traditional herb medicine to treat rheumatic arthritis and gynecological diseases. In this study, a novel polysaccharide (MDP1) with a molecular weight of 139.54 kDa was isolated from Millettia dielsiana by DEAE-52 cellulose chromatography. Its sulfated derivative (S-MDP1) was prepared by the chlorosulfonic acid-pyridine method. Structural analysis indicated that MDP1 was a pectic polysaccharide and did not exhibit a triple helical conformation. In vitro, MDP1 and S-MDP1 had protective effect on HO-induced PC12 cells death. Immunological assay showed that MDP1 and S-MDP1 significantly stimulated NO production and phagocytic activity in RAW264.7 cells, and promoted lymphocyte proliferation. Anticoagulant assay indicated that S-MDP1 effectively prolonged activated partial thromboplastin time (APTT) and thrombin time (TT), but not prothrombin time (PT), and MDP1 prolonged APTT and TT weakly. These results implied that MDP1 and S-MDP1 had the potential to be used as antioxidant, immunomodulatory and anticoagulant agents.
美丽崖豆藤是一种传统草药,用于治疗风湿性关节炎和妇科疾病。在本研究中,通过 DEAE-52 纤维素层析从美丽崖豆藤中分离出一种新型多糖(MDP1),其分子量为 139.54 kDa。采用氯磺酸-吡啶法制备其硫酸化衍生物(S-MDP1)。结构分析表明,MDP1 是一种果胶多糖,不具有三螺旋构象。体外实验表明,MDP1 和 S-MDP1 对 HO 诱导的 PC12 细胞死亡具有保护作用。免疫测定表明,MDP1 和 S-MDP1 可显著刺激 RAW264.7 细胞中 NO 的产生和吞噬活性,并促进淋巴细胞增殖。抗凝试验表明,S-MDP1 可有效延长活化部分凝血活酶时间(APTT)和凝血酶时间(TT),但不延长凝血酶原时间(PT),而 MDP1 则可微弱延长 APTT 和 TT。这些结果表明,MDP1 和 S-MDP1 具有作为抗氧化剂、免疫调节剂和抗凝剂的潜力。