IRD, APHM, MEPHI, IHU Méditerranée Infection, Aix-Marseille Université, Marseille, France.
Clinical Microbiology Laboratory, Faculty of Medicine and Medical Sciences, University of Balamand, PO Box: 33, Amioun, Lebanon.
Microbiome. 2018 May 24;6(1):94. doi: 10.1186/s40168-018-0485-5.
After a decade of research and metagenomic analyses, our knowledge of the human microbiota appears to have reached a plateau despite promising results. In many studies, culture has proven to be essential in describing new prokaryotic species and filling metagenomic gaps. In 2015, only 2172 different prokaryotic species were reported to have been isolated at least once from the human body as pathogens or commensals. In this review, we update the previous repertoire by reporting the different species isolated from the human body to date, increasing it by 28% to reach a total of 2776 species associated with human beings. They have been classified into 11 different phyla, mostly the Firmicutes, Proteobacteria, Bacteroidetes, and Actinobacteria. Finally, culturomics contributed up to 66.2% towards updating this repertoire by reporting 400 species, of which 288 were novel. This demonstrates the need to continue the culturing work, which seems essential in order to decipher the hidden human microbial content.
经过十年的研究和宏基因组分析，尽管取得了有希望的结果，但我们对人类微生物组的了解似乎已经达到了一个瓶颈。在许多研究中，培养已被证明对于描述新的原核生物物种和填补宏基因组空白至关重要。2015 年，据报道，仅从人体中分离出至少一次的致病性或共生性的不同原核生物物种就有 2172 种。在这篇综述中，我们通过报告迄今为止从人体中分离出的不同物种来更新以前的目录，将其增加了 28%，达到了总共 2776 种与人类相关的物种。它们被分类为 11 个不同的门，主要是厚壁菌门、变形菌门、拟杆菌门和放线菌门。最后，培养组学通过报告 400 种新物种（其中 288 种是新物种），对更新这个目录的贡献高达 66.2%。这表明需要继续进行培养工作，这似乎对于解析隐藏的人类微生物组内容至关重要。
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