Davidson Rebecca M, DeGroote Mary Ann, Marola Jamie L, Buss Sarah, Jones Victoria, McNeil Michael R, Freifeld Alison G, Elaine Epperson L, Hasan Nabeeh A, Jackson Mary, Iwen Peter C, Salfinger Max, Strong Michael
Center for Genes, Environment and Health, National Jewish Health, Denver, CO 80206, USA.
Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA.
Int J Syst Evol Microbiol. 2017 Aug;67(8):2640-2645. doi: 10.1099/ijsem.0.001998. Epub 2017 Aug 15.
A novel slowly growing, non-chromogenic species of the class Actinobacteria was isolated from a human respiratory sample in Nebraska, USA, in 2012. Analysis of the internal transcribed spacer sequence supported placement into the genus Mycobacterium with high sequence similarity to a previously undescribed strain isolated from a patient respiratory sample from Oregon, USA, held in a collection in Colorado, USA, in 2000. The two isolates were subjected to phenotypic testing and whole genome sequencing and found to be indistinguishable. The bacteria were acid-fast stain-positive, rod-shaped and exhibited growth after 7-10 days on solid media at temperatures ranging from 25 to 42°C. Colonies were non-pigmented, rough and slightly raised. Analyses of matrix-assisted laser desorption ionization time-of-flight profiles showed no matches against a reference library of 130 mycobacterial species. Full-length 16S rRNA gene sequences were identical for the two isolates, the average nucleotide identity (ANI) between their genomes was 99.7 % and phylogenetic comparisons classified the novel mycobacteria as the basal most species in the slowly growing Mycobacterium clade. Mycobacterium avium is the most closely related species based on rpoB gene sequence similarity (92 %), but the ANI between the genomes was 81.5 %, below the suggested cut-off for differentiating two species (95 %). Mycolic acid profiles were more similar to M. avium than to Mycobacterium simiae or Mycobacterium abscessus. The phenotypic and genomic data support the conclusion that the two related isolates represent a novel Mycobacterium species for which the name Mycobacterium talmoniae sp. nov. is proposed. The type strain is NE-TNMC-100812T (=ATCC BAA-2683T=DSM 46873T).
2012年，从美国内布拉斯加州的一份人类呼吸道样本中分离出一种新型的、生长缓慢的、非产色放线菌纲细菌。对内部转录间隔区序列的分析支持将其归入分枝杆菌属，该序列与2000年保存在美国科罗拉多州一个菌种保藏中心的、从美国俄勒冈州一名患者呼吸道样本中分离出的一株先前未描述的菌株具有高度序列相似性。对这两株分离株进行了表型测试和全基因组测序，发现它们无法区分。这些细菌抗酸染色呈阳性，杆状，在25至42°C的温度下，在固体培养基上培养7 - 10天后生长。菌落无色素，粗糙且稍有凸起。基质辅助激光解吸电离飞行时间质谱分析结果与130种分枝杆菌参考文库中的菌株均不匹配。两株分离株的全长16S rRNA基因序列相同，它们基因组之间的平均核苷酸同一性（ANI）为99.7%，系统发育比较将这种新型分枝杆菌归类为生长缓慢的分枝杆菌进化枝中最基部的物种。基于rpoB基因序列相似性（92%），鸟分枝杆菌是最密切相关的物种，但它们基因组之间的ANI为81.5%，低于区分两个物种的建议阈值（95%）。分枝菌酸谱与鸟分枝杆菌比与猿分枝杆菌或脓肿分枝杆菌更相似。表型和基因组数据支持以下结论：这两株相关分离株代表一种新型分枝杆菌物种，为此提出新名称塔尔蒙分枝杆菌（Mycobacterium talmoniae sp. nov.）。模式菌株为NE-TNMC-100812T（=ATCC BAA-2683T=DSM 46873T）。
