Yang Jiajun, Shang Peng, Liu Zongliang, Wang Jing, Zhang Bo, Zhang Hao
School of Animal Husbandry and Veterinary Medicine, Jiangsu Vocational College of Agriculture and Forestry, Jurong, Jiangsu, China.
Beijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural University, Beijing, China.
Front Immunol. 2025 Apr 10;16:1540548. doi: 10.3389/fimmu.2025.1540548. eCollection 2025.
The aim of this study was to elucidate the intrinsic microbiota residing in the heart and liver, which was enriched with Ligilactobacillus salivarius supplementation and its roles in defending anti-oxidation of heat stress. The specific pathogen free (SPF) mice were employed to perform the study. Genomic sequencing showed that the intrinsic microbes in the heart and liver of SPF mice, which were primarily of the genera and , functioned in organic metabolism, environmental information processing, cellular processes, and genetic information processing. sp. were found in the liver but not in the heart. The heart had a lower bacterial abundance than the liver. A culturomic assay of the heart flushing liquid indicated that the dominant species of bacteria were sp._3PA37C10, , unclassified g , and unclassified p . Intrinsic bacteria exist in the heart due to their inhibitory action against pathogenic Escherichia coli. After, the mice were supplemented with to optimize the microbiota levels. The dominant bacterial phyla in the liver and heart were Bacillota, Bacteroidota, Pseudomonadota, Thermodesulfobacteriota, andActinomycetota, which comprised 98.2% of total bacteria. The genus was also abundant. Core bacteria such as are translocated from the intestine to the heart and liver. The enriched bacterial composition up-regulated anti-oxidation capacities in the heart and liver. The levels of reactive oxygen species and superoxide dismutase (SOD) were significantly improved compared to those in control (P < 0.01). In conclusion, intrinsic bacteria present in the heart and liver alleviate infection by pathogens, environmental and genetic information processing, and cellular processes during heat stress exposure. Diet with supplementation regulated the translocation of core bacteria to the heart and liver, improved bacterial composition, and induced a higher anti-oxidative capacity under heat stress.
本研究的目的是阐明心脏和肝脏中存在的固有微生物群，其通过唾液乳杆菌补充剂得以富集，以及其在抵御热应激抗氧化方面的作用。使用无特定病原体（SPF）小鼠进行该研究。基因组测序表明，SPF小鼠心脏和肝脏中的固有微生物主要属于 属和 属，在有机代谢、环境信息处理、细胞过程和遗传信息处理中发挥作用。在肝脏中发现了 菌，但在心脏中未发现。心脏中的细菌丰度低于肝脏。对心脏冲洗液进行的培养组学分析表明，优势细菌种类为 菌_sp.3PA37C10、 菌、未分类的_g 菌和未分类的_p_ 菌。由于其对致病性大肠杆菌的抑制作用，心脏中存在固有细菌。之后，给小鼠补充 以优化微生物群水平。肝脏和心脏中的优势细菌门为芽孢杆菌门、拟杆菌门、假单胞菌门、热脱硫杆菌门和放线菌门，它们占细菌总数的98.2%。 属也很丰富。诸如 菌等核心细菌从肠道转移至心脏和肝脏。富集的细菌组成上调了心脏和肝脏中的抗氧化能力。与对照组相比，活性氧和超氧化物歧化酶（SOD）水平显著提高（P < 0.01）。总之，心脏和肝脏中存在的固有细菌在热应激暴露期间减轻病原体感染、环境和遗传信息处理以及细胞过程。补充 的饮食调节了核心细菌向心脏和肝脏的转移，改善了细菌组成，并在热应激下诱导了更高的抗氧化能力。
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