Institute of Environmental Health, College of Public Health, National Taiwan University, Taipei 10055, Taiwan.
Institute of Occupational Medicine and Industrial Hygiene, College of Public Health, National Taiwan University, Taipei 10055, Taiwan.
Int J Environ Res Public Health. 2018 May 24;15(6):1066. doi: 10.3390/ijerph15061066.
Perfluoroalkyl substances (PFASs) are stable and persistent in the environment, animals, and humans. PFASs can penetrate placenta and affect fetal growth. We investigated associations between prenatal exposure to perfluorooctanoic acid (PFOA), perfluorooctyl sulfonate (PFOS), perfluorononanoic acid (PFNA), and perfluoroundecanoic acid (PFUA) and global methylation levels. Specific Aims and Methods: The study used the subjects from Taiwan Birth Panel birth cohort study, including all pregnant women who gave birth between July 2004 and June 2005 in four hospitals in Taipei city and New Taipei City. A total of 363 mother-infant pairs were included in the final analyses. PFOA, PFOS, PFNA, and PFUA were measured by UPLC-MS/MS in cord blood. LINE-1 and Alu repeated elements from cord blood was used to represent global DNA methylation levels. Multivariable regression models were used to adjust potential confounders.
After controlling for potential confounders, each unit increase in the natural log-transformed PFOS exposure was associated with an adjusted OR of 1.72 (95% CI: 1.03, 2.88) for low Alu methylation level when dichotomized methylation level by medium. No significant effects between PFOA, PFNA, PFUA and methylation levels in the multivariable regression models were observed.
Our findings suggest that prenatal PFOS exposure may be associated with low Alu methylation level.
全氟烷基物质（PFASs）在环境、动物和人类中稳定且持久。PFASs 可以穿透胎盘并影响胎儿生长。我们研究了产前暴露于全氟辛酸（PFOA）、全氟辛烷磺酸（PFOS）、全氟壬酸（PFNA）和全氟癸酸（PFUA）与全球甲基化水平之间的关联。
本研究使用了来自台湾出生队列研究的受试者，包括 2004 年 7 月至 2005 年 6 月在台北市和新北市四家医院分娩的所有孕妇。共有 363 对母婴对纳入最终分析。通过超高效液相色谱-串联质谱法（UPLC-MS/MS）在脐带血中测量 PFOA、PFOS、PFNA 和 PFUA。使用脐带血中的 LINE-1 和 Alu 重复元件来代表全球 DNA 甲基化水平。多变量回归模型用于调整潜在的混杂因素。
在控制了潜在的混杂因素后，PFOS 暴露的自然对数值每增加一个单位，与低 Alu 甲基化水平的调整比值比（OR）为 1.72（95%置信区间：1.03，2.88），当按中位数将甲基化水平二分法时。在多变量回归模型中，PFOA、PFNA 和 PFUA 与甲基化水平之间没有观察到显著的影响。
我们的研究结果表明，产前 PFOS 暴露可能与低 Alu 甲基化水平有关。
