Department of Clinical Nutrition, Institute of Public Health and Clinical Nutrition, University of Eastern Finland, Kuopio, Finland.
Department of Chronic Disease Prevention, National Institute for Health and Welfare, Helsinki, Finland.
Nutr Diabetes. 2018 May 25;8(1):35. doi: 10.1038/s41387-018-0046-9.
We recently reported using non-targeted metabolic profiling that serum indolepropionic acid (IPA), a microbial metabolite of tryptophan, was associated with a lower likelihood of developing type 2 diabetes (T2D). In the present study, we established a targeted quantitative method using liquid chromatography with mass spectrometric detection (HPLC-QQQ-MS/MS) and measured the serum concentrations of IPA in all the participants from the Finnish Diabetes Prevention Study (DPS), who had fasting serum samples available from the 1-year study follow-up (n = 209 lifestyle intervention and n = 206 control group). Higher IPA at 1-year study was inversely associated with the incidence of T2D (OR [CI]: 0.86 [0.73-0.99], P = 0.04) and tended to be directly associated with insulin secretion (β = 0.10, P = 0.06) during the mean 7-year follow-up. Moreover, IPA correlated positively with dietary fiber intake (g/day: r = 0.24, P = 1 × 10) and negatively with hsCRP concentrations at both sampling (r = - 0.22, P = 0.0001) and study follow-up (β = - 0.19, P = 0.001). Thus, we suggest that the putative effect of IPA on lowering T2D risk might be mediated by the interplay between dietary fiber intake and inflammation or by direct effect of IPA on β-cell function.
我们最近报道了使用非靶向代谢组学方法发现血清吲哚丙酸（IPA）与较低的 2 型糖尿病（T2D）发病风险相关，IPA 是色氨酸的微生物代谢产物。在本研究中，我们建立了一种使用液相色谱-串联质谱检测（HPLC-QQQ-MS/MS）的靶向定量方法，并测量了芬兰糖尿病预防研究（DPS）中所有参与者的血清 IPA 浓度，这些参与者在 1 年的研究随访中都有空腹血清样本（n=209 例生活方式干预组和 n=206 例对照组）。1 年研究时 IPA 浓度较高与 T2D 的发生呈负相关（OR[CI]：0.86[0.73-0.99]，P=0.04），且在平均 7 年随访期间与胰岛素分泌呈正相关（β=0.10，P=0.06）。此外，IPA 与膳食纤维摄入量呈正相关（g/天：r=0.24，P=1×10），与 hsCRP 浓度呈负相关（r=−0.22，P=0.0001）和研究随访时（β=−0.19，P=0.001）。因此，我们认为 IPA 降低 T2D 风险的潜在作用可能是通过膳食纤维摄入和炎症之间的相互作用或 IPA 对β细胞功能的直接作用介导的。
