Bellardita Carmelo, Marcantoni Maite, Löw Peter, Kiehn Ole
Department of Neuroscience, University of Copenhagen, Copenhagen, Denmark.
Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden.
Bio Protoc. 2018 Apr 5;8(7):e2784. doi: 10.21769/BioProtoc.2784.
Spinal cord injury (SCI) is characterized by multiple sensory/motor impairments that arise from different underlying neural mechanisms. Linking specific sensory/motor impairments to neural mechanism is limited by a lack of direct experimental access to these neural circuits. Here, we describe an experimental model which addresses this shortcoming. We generated a mouse model of chronic spinal cord injury that reliably reproduces spasticity observed after SCI, while at the same time allows study of motor impairments and in an preparation of the spinal cord. The model allows for the combination of mouse genetics in and conditions with advanced imaging, behavioral analysis, and detailed electrophysiology, techniques which are not easily applied in conventional SCI models.
脊髓损伤（SCI）的特征是由不同的潜在神经机制引起的多种感觉/运动障碍。由于缺乏对这些神经回路的直接实验通路，将特定的感觉/运动障碍与神经机制联系起来受到限制。在此，我们描述了一种解决这一缺陷的实验模型。我们构建了一种慢性脊髓损伤小鼠模型，该模型能可靠地重现脊髓损伤后观察到的痉挛，同时还能在脊髓制备中研究运动障碍。该模型允许在体内和体外条件下将小鼠遗传学与先进的成像、行为分析及详细的电生理学相结合，而这些技术在传统的脊髓损伤模型中不易应用。
