Broda H, Gooch V D, Taylor W, Aiuto N, Hastings J W
Department of Cellular and Developmental Biology, Harvard University, Cambridge, Massachusetts 02138.
J Biol Rhythms. 1986 Fall;1(3):251-63. doi: 10.1177/074873048600100307.
During measurements of the circadian (approximately 24-hr) rhythms of spontaneous bioluminescence in the marine dinoflagellate Gonyaulax polyedra, the individual cultures in vials were shielded from otherwise constant dim light for 1-3 min every 20-60 min by a photomultiplier housing that was moved from vial to vial. The high-frequency dark pulses caused a small but consistent shortening of the free-running circadian period, but there was no indication that the dark pulses caused entrainment. Hardware and software components of the microcomputer-controlled data collection system are described. A microcomputer controlled the movement of the photomultiplier and acquired the data via an analog-to-digital converter. The algorithms distinguished and separately recorded background glow, intermittent flashes, and total light from populations ranging in number from 10(3) to 10(5) cells in volumes from 1 to 10 ml. Fast video display techniques allowed continuous on-line viewing of incoming data, together with a display of the data recorded over the preceding day or two. Detection of mechanical and software errors coupled with recovery systems maintained high reliability of data collection.
在测量海洋甲藻多甲藻（Gonyaulax polyedra）自发生物发光的昼夜节律（约24小时）时，小瓶中的单个培养物每隔20 - 60分钟会被从一个小瓶移到另一个小瓶的光电倍增管罩遮蔽1 - 3分钟，使其免受原本恒定的弱光照射。高频暗脉冲导致自由运行的昼夜周期出现轻微但持续的缩短，但没有迹象表明暗脉冲会引起昼夜节律的调整。文中描述了微计算机控制的数据采集系统的硬件和软件组件。一台微型计算机控制光电倍增管的移动，并通过模数转换器获取数据。这些算法能够区分并分别记录背景光、间歇性闪光以及来自数量在10³至10⁵个细胞、体积在1至10毫升范围内的群体的总光量。快速视频显示技术允许对传入数据进行连续在线查看，同时显示前一两天记录的数据。机械和软件错误检测以及恢复系统保证了数据采集的高可靠性。
