Donnelly J J
Department of Ophthalmology, Scheie Eye Institute, School of Medicine, University of Pennsylvania, Philadelphia 19104.
Autoimmunity. 1988;1(3):207-16. doi: 10.3109/08916938808997165.
Hartley guinea pigs were injected with Onchocerca lienalis microfilariae (Mf) as a model for human infection with Onchocerca volvulus. Subconjunctival injection of guinea pigs with O. lienalis Mf results in penetration of Mf to the central cornea, and formation of acute inflammatory reactions resembling those of human onchocerciasis around dead Mf. Sera from these animals contain IgG autoantibodies directed against at least two components of 3M KC1 extracts of guinea pig cornea and liver. Components with apparent molecular weights of 94-99 and 110-120 kilodaltons (KD) were recognized in both cornea and liver extracts, while 100, 74 and 34 kD components were recognized in liver extracts only. Components of 140 kD and 84 kD were recognized in cornea extracts only. The guinea pig liver extract cross-reacted with homogenate antigens from O. lienalis Mf, while the guinea pig cornea extract did not. Autoantibody-mediated inflammatory mechanisms may contribute to immunopathologic processes in onchocerciasis.
将盘尾丝虫微小丝蚴（Mf）注射到哈特利豚鼠体内，以此作为人类感染盘尾丝虫的模型。将盘尾丝虫Mf结膜下注射到豚鼠体内后，Mf会穿透至角膜中央，并在死亡的Mf周围形成类似于人类盘尾丝虫病的急性炎症反应。这些动物的血清中含有针对豚鼠角膜和肝脏3M KCl提取物至少两种成分的IgG自身抗体。在角膜和肝脏提取物中均识别出表观分子量为94 - 99千道尔顿（KD）和110 - 120千道尔顿的成分，而仅在肝脏提取物中识别出100、74和34 kD的成分。仅在角膜提取物中识别出140 kD和84 kD的成分。豚鼠肝脏提取物与盘尾丝虫Mf的匀浆抗原发生交叉反应，而豚鼠角膜提取物则未发生。自身抗体介导的炎症机制可能有助于盘尾丝虫病的免疫病理过程。
