Donnelly J J, Xi M S, Haldar J P, Hill D E, Lok J B, Khatami M, Rockey J H
Department of Ophthalmology, Scheie Eye Institute, School of Medicine, University of Pennsylvania, Philadelphia 19104.
Invest Ophthalmol Vis Sci. 1988 May;29(5):827-31.
Hartley guinea pigs were injected with microfilariae (Mf) of Onchocerca lienalis as a model for acute inflammatory responses to Mf in human Onchocerca volvulus infection. IgG autoantibody reactive with a 3 M KCl extract of guinea pig cornea was detected by ELISA in the serum of guinea pigs injected with O. lienalis Mf three or more times sub-conjunctivally, or two or more times subcutaneously. Administration of the microfilaricides diethylcarbamazine citrate and ivermectin did not alter the proportion of animals expressing autoantibody or the mean autoantibody titer. The severity of acute corneal inflammatory reactions to Mf was similar in animals with and without circulating autoantibody. Although autoantibody responses did not correlate with acute corneal inflammatory reactions to dead Mf, the ability of Mf to induce formation of an antibody reactive with a component of autologous cornea suggests that autoimmune mechanisms might participate in chronic onchocercal lesions in the cornea, eg, sclerosing keratitis.
将盘尾丝虫的微丝蚴（Mf）注射到哈特利豚鼠体内，作为人类盘尾丝虫感染中对Mf急性炎症反应的模型。通过ELISA法在结膜下注射3次或更多次盘尾丝虫Mf，或皮下注射2次或更多次的豚鼠血清中，检测到与豚鼠角膜3M KCl提取物反应的IgG自身抗体。给予微丝蚴杀虫剂枸橼酸乙胺嗪和伊维菌素，并不会改变表达自身抗体的动物比例或平均自身抗体滴度。在有和没有循环自身抗体的动物中，对Mf的急性角膜炎症反应的严重程度相似。虽然自身抗体反应与对死亡Mf的急性角膜炎症反应无关，但Mf诱导形成与自体角膜成分反应的抗体的能力表明，自身免疫机制可能参与角膜的慢性盘尾丝虫病病变，如硬化性角膜炎。
