School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China.
Food Funct. 2018 Jun 20;9(6):3313-3320. doi: 10.1039/c8fo00021b.
Bioactive peptides derived from mussels have multiple healthcare functions. Herein, we aimed to examine the effects of mussel peptide preparation on lifespan, stress resistance, apoptosis and aging in Caenorhabditis elegans. Lifespan was determined by counting the number of surviving nematodes daily. ROS level and lipofuscin were measured using a fluorescent microscope. The mussel protein was prepared and hydrolyzed, and then fractionated by ultrafiltration. The fraction (<3 kDa) was purified by gel filtration to obtain the bioactive peptides, and the peptide sequences included in the fractions were identified, which were mostly composed of peptides with <20 amino acid residues. Mussel peptides treatment was found to significantly increase oxidative stress resistance and extend the lifespan of C. elegans. Moreover, this treatment also reduced endogenous ROS level and aging pigments accumulation in C. elegans as well as apoptosis. Collectively, these findings demonstrated that mussel peptides could contribute to healthspan extension of C. elegans through regulating mRNA expression of daf-2 and daf-16. These results highlighted the important role of mussel peptides for food and pharmaceutical industries to develop new nutraceuticals and functional foods.
从贻贝类中提取的生物活性肽具有多种保健功能。本研究旨在探讨贻贝肽制剂对秀丽隐杆线虫寿命、应激抗性、凋亡和衰老的影响。通过每天计数存活线虫的数量来确定寿命。使用荧光显微镜测量 ROS 水平和脂褐素。制备贻贝蛋白并进行水解，然后通过超滤进行分级。<3 kDa 的级分通过凝胶过滤进行纯化以获得生物活性肽，鉴定出包含在级分中的肽序列，它们主要由<20 个氨基酸残基的肽组成。贻贝肽处理可显著提高秀丽隐杆线虫的氧化应激抗性并延长其寿命。此外，该处理还降低了秀丽隐杆线虫内源性 ROS 水平和衰老色素的积累以及凋亡。综上所述，这些发现表明贻贝肽可通过调节 daf-2 和 daf-16 的 mRNA 表达来延长秀丽隐杆线虫的健康寿命。这些结果强调了贻贝肽在食品和制药行业开发新型营养保健品和功能性食品中的重要作用。
