Priya Kumutha, Sulaiman Joanita, How Kah Yan, Yin Wai-Fong, Chan Kok-Gan
International Genome Centre, Jiangsu University, Zhenjiang, China.
Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, 50603, Malaysia.
Arch Microbiol. 2018 Sep;200(7):1135-1142. doi: 10.1007/s00203-018-1526-y. Epub 2018 May 23.
Quorum sensing (QS) is a term used to describe cell-to-cell communication that enables bacteria to orchestrate group behaviours according to density of bacterial cells. In Gram-negative bacteria, this signalling system is widely known to regulate a variety of different phenotypes such as antibiotic production and biofilm formation. In this study, we report the production of N-acyl homoserine lactones produced by Chromobacterium haemolyticum strain KM2, a bacterium isolated from a river water of a reserved tropical national park. Preliminary screening of QS activity using biosensor reporter assays indicated that C. haemolyticum strain KM2 produces both short- and long-chain AHLs. Analysis with high-resolution liquid chromatography-mass spectrometry (LC-MS/MS) analysis revealed the production of three AHLs by strain KM2: N-octanoyl-L-homoserine lactone (C8-HSL), N-dodecanoyl-L-homoserine lactone (C12-HSL), and N-3-oxo-dodecanoyl-L-homoserine lactone (OC12-HSL). This bacterial isolate also exhibited strong β-haemolytic activity. To the best of our knowledge, this is the first documentation of QS activity and multiple AHLs production by C. haemolyticum strain KM2.
群体感应（QS）是一个用于描述细胞间通讯的术语，它使细菌能够根据细菌细胞的密度来协调群体行为。在革兰氏阴性细菌中，这种信号系统广为人知，可调节多种不同的表型，如抗生素生产和生物膜形成。在本研究中，我们报告了从一个热带国家自然保护区的河水中分离出的溶血色杆菌KM2菌株产生的N-酰基高丝氨酸内酯。使用生物传感器报告分析对QS活性进行初步筛选表明，溶血色杆菌KM2菌株同时产生短链和长链AHLs。通过高分辨率液相色谱-质谱联用（LC-MS/MS）分析发现，KM2菌株产生了三种AHLs：N-辛酰-L-高丝氨酸内酯（C8-HSL）、N-十二酰-L-高丝氨酸内酯（C12-HSL）和N-3-氧代-十二酰-L-高丝氨酸内酯（OC12-HSL）。这种细菌分离株还表现出很强的β-溶血活性。据我们所知，这是关于溶血色杆菌KM2菌株QS活性和多种AHLs产生的首次记录。
