Escola Universitària Salesiana de Sarrià, Pg. Sant Joan Bosco 74, 08017,, Barcelona, Spain.
Biomaterials, Biomechanics and Tissue Engineering group, Technical University of Catalonia, Pav. E, Av. Diagonal 647,, Barcelona, Spain.
J Mater Sci Mater Med. 2018 May 23;29(6):73. doi: 10.1007/s10856-018-6082-0.
Osseointegration of implants is conversely related to the generation of a fibrous tissue capsule around the implant by the host environment. Although TGF-β1 plays many roles in regeneration processes, it is the cytokine to be mostly associated to the production of fibrotic tissue and thus, its inhibition has demonstrated to be beneficial to prevent several fibrotic reactions. Surface biofunctionalization enables the immobilization of biologically active molecules on an implant surface to tailor the biological response of the host. Here, we studied in vitro biological effects of biofunctionalized CP-Ti surfaces with a TGF-β1 inhibitor peptide, P144. A reliable biofunctionalization process that tethers P144 peptides to commercially pure titanium was developed. Differentiation of human mesenchymal stem cells, osteoblasts and fibroblasts on P144-functionalized and control surfaces was assessed at the gene expression and protein production levels. Results showed that P144-functionalized surfaces reduced expression and production of fibrotic differentiation markers and increased osteoblastic differentiation markers. Therefore, biofunctionalization of surfaces with TGF-β1 inhibitor peptides are an alternative promising strategy for inducing osseointegration around medical devices and implants.
种植体的骨整合与宿主环境在种植体周围产生纤维组织囊相反。虽然 TGF-β1 在再生过程中发挥多种作用，但它是与纤维组织产生最相关的细胞因子，因此，抑制 TGF-β1 已被证明有益于预防多种纤维反应。表面生物功能化可使生物活性分子固定在植入物表面，从而调整宿主的生物学反应。在这里，我们研究了具有 TGF-β1 抑制剂肽 P144 的 CP-Ti 表面的体外生物学效应。开发了一种可靠的将 P144 肽键合到商用纯钛的生物功能化方法。在基因表达和蛋白质产生水平上评估了人骨髓间充质干细胞、成骨细胞和成纤维细胞在 P144 功能化和对照表面上的分化情况。结果表明，P144 功能化表面降低了纤维化分化标志物的表达和产生，并增加了成骨细胞分化标志物的表达和产生。因此，用 TGF-β1 抑制剂肽对表面进行生物功能化是一种有前途的替代策略，可诱导医疗器械和植入物周围的骨整合。
