Integrated Laboratory of Plant Biology, Department of Botany, Institute of Biosciences, Federal University of Rio de Janeiro State, UNIRIO. Av. Pasteur, 458, Urca, Rio de Janeiro, 22290-240, Brazil.
Laboratory of Protein Biochemistry, Center of Innovation of Mass Spectrometry, Federal University of Rio de Janeiro State, UNIRIO. Av. Pasteur, 296, Urca, Rio de Janeiro, 22290-250, Brazil.
Plant Cell Rep. 2018 Aug;37(8):1173-1185. doi: 10.1007/s00299-018-2303-8. Epub 2018 May 23.
Gibberellic acid elicited synthesis of many phenols from different classes and enhanced production of sesquiterpenoids, polyterpenoids, steroids and monoterpenoids compared to control and 6-benzylaminopurine. Little is known about the effects of 6-benzylaminopurine (BA) and gibberellic acid (GA3) on the synthesis of secondary metabolites in species of Lamiaceae. In this study, for the first time, the profile of secondary metabolites in plantlets of Cunila menthoides was characterized, using UPLC-ESI-Qq-oaTOF-MS. Ninety metabolites were identified, including polyphenols and terpenes. BA down-regulated most of the identified molecules in relation to GA3 and MS0 (control). The results showed that GA3 elicited synthesis of many phenols from different classes, and seemed to play a major role in the shikimate pathway in relation to BA. GA3 enhanced production of sesquiterpenoids, polyterpenoids, steroids and monoterpenoids compared to MS0 and BA, and also seemed to positively influence the MEP/DOXP and MVA pathways. These data show the most comprehensive metabolomic profile of Cunila menthoides to date, and the effects of BA and GA3 on the synthesis of secondary metabolites, modulating quantitative aspects of metabolism in Lamiaceae.
赤霉素（GA3）与 6-苄基氨基嘌呤（BA）相比，能从不同类别中诱导合成许多酚类物质，并促进倍半萜、多萜、甾体和单萜的产生。关于 6-苄基氨基嘌呤（BA）和赤霉素（GA3）对唇形科植物次生代谢产物合成的影响，目前知之甚少。本研究首次采用 UPLC-ESI-Qq-oaTOF-MS 对 Mentha canadensis 组培苗的次生代谢产物进行了特征分析。共鉴定出 90 种代谢产物，包括多酚和萜类化合物。BA 下调了与 GA3 和 MS0（对照）相比大多数已鉴定的分子。结果表明，GA3 从不同类别中诱导合成了许多酚类物质，并且似乎在莽草酸途径中与 BA 相比发挥了主要作用。与 MS0 和 BA 相比，GA3 能促进倍半萜、多萜、甾体和单萜的产生，同时似乎也能积极影响 MEP/DOXP 和 MVA 途径。这些数据显示了迄今为止对 Mentha canadensis 最全面的代谢组学特征，以及 BA 和 GA3 对次生代谢产物合成的影响，调节了唇形科植物代谢的定量方面。
