Petrova E S, Isaeva E N, Kolos E A, Korzhevskii D E
Laboratory of Functional Morphology of the Central and Peripheral Nervous System, Department of General and Special Morphology, Research Institute of Experimental Medicine, St. Petersburg, Russia.
Bull Exp Biol Med. 2018 May;165(1):161-165. doi: 10.1007/s10517-018-4120-z. Epub 2018 May 24.
Quantitative analysis of blood vessels in the distal segment of rat sciatic nerve after its ligation for 40 sec and subperineurial administration of mesenchymal stem cells or dissociated cells of rat embryonic spinal cord was carried our by immunohistochemical tracing of von Willebrand factor, a marker of endothelial cells of blood vessels. It was found that the number of blood vessels per unit area of the nerve trunk in 21 days after injury and administration of mesenchymal stem cells increased by more than 1.5 times in comparison with the control (damaged nerve). After administration of dissociated cells of the embryonic spinal cord, this effect was not observed. It is assumed that mesenchymal stem cells stimulate the growth of vessels of the damaged nerve via production of angiogenic factors.
通过对血管内皮细胞标志物血管性血友病因子进行免疫组织化学追踪，对大鼠坐骨神经结扎40秒后并在神经束膜下给予间充质干细胞或大鼠胚胎脊髓解离细胞的大鼠坐骨神经远端节段中的血管进行定量分析。结果发现，与对照组（损伤神经）相比，损伤后21天给予间充质干细胞后，神经干单位面积内的血管数量增加了1.5倍以上。给予胚胎脊髓解离细胞后，未观察到这种效果。推测间充质干细胞通过产生血管生成因子来刺激受损神经的血管生长。
