Petrova Ye S, Isayeva Ye N
Morfologiia. 2015;148(4):7-11.
The study was conducted on 6 female and 36 male adult Wistar rats to compare the effects of dissociated cells derived from different embryonic CNS anlages, on the growth of regenerating nerve fibers in the damaged nerve of the recipient. After the sciatic nerve was damaged by ligation, part of the animals received the injection into the proximal portion of the nerve with a suspension of the cells obtained by dissociation of the fragments of spinal cord or forebrain vesicle taken from rat embryos at Day 15 of development. The analysis of transverse semithin sections of the distal part of the nerves was performed 21 and 60 days after surgery. It was found that the number of regenerating myelinated nerve fibers was increased 60 days after the injection of dissociated embryonic spinal cord cells, but not the neocortical cells into the damaged nerve of the recipient.
该研究以6只成年雌性和36只成年雄性Wistar大鼠为对象，比较源自不同胚胎中枢神经系统原基的离散细胞，对受体受损神经中再生神经纤维生长的影响。在坐骨神经通过结扎造成损伤后，部分动物在神经近端注射取自发育第15天大鼠胚胎的脊髓或前脑泡碎片经解离后获得的细胞悬液。术后21天和60天对神经远端的横向半薄切片进行分析。结果发现，在向受体受损神经注射离散的胚胎脊髓细胞而非新皮质细胞后60天，再生有髓神经纤维的数量增加。
